RT Journal Article
T1 Involvement of Burkholderiaceae and sulfurous volatiles in disease-suppressive soils
A1 Carrión Bravo, Víctor José
A1 Cordovez, Viviane
A1 Tyc, Olaf
A1 Etalo, Desalegn W
A1 de Bruijn, Irene
A1 de Jager, Victor CL
A1 Medema, Marnix
A1 Eberl, Leo
A1 Raaijmakers, Jos M
K1 Ecología microbiana de suelos
K1 Compuestos orgánicos volátiles
K1 Rhizoctonia solani
AB Disease-suppressive soils are ecosystems in which plants suffer less from root infections due to the activities of specificmicrobial consortia. The characteristics of soils suppressive to specific fungal root pathogens are comparable to those ofadaptive immunity in animals, as reported by Raaijmakers and Mazzola (Science 352:1392–3, 2016), but the mechanismsand microbial species involved in the soil suppressiveness are largely unknown. Previous taxonomic and metatranscriptomeanalyses of a soil suppressive to the fungal root pathogen Rhizoctonia solani revealed that members of the Burkholderiaceaefamily were more abundant and more active in suppressive than in non-suppressive soils. Here, isolation, phylogeny, andsoil bioassays revealed a significant disease-suppressive activity for representative isolates of Burkholderia pyrrocinia,Paraburkholderia caledonica, P. graminis, P. hospita, and P. terricola. In vitro antifungal activity was only observed forP. graminis. Comparative genomics and metabolite profiling further showed that the antifungal activity of P. graminis PHS1was associated with the production of sulfurous volatile compounds encoded by genes not found in the other four genera.Site-directed mutagenesis of two of these genes, encoding a dimethyl sulfoxide reductase and a cysteine desulfurase, resultedin a loss of antifungal activity both in vitro and in situ. These results indicate that specific members of the Burkholderiaceaefamily contribute to soil suppressiveness via the production of sulfurous volatile compounds.
PB Oxford University Press
YR 2018
FD 2018-06-13
LK https://hdl.handle.net/10630/33949
UL https://hdl.handle.net/10630/33949
LA eng
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026