RT Journal Article
T1 RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi
A1 Padilla-Roji, Isabel
A1 Ruiz-Jimenez, Laura
A1 Bakhat, Nisrine
A1 Vielba-Fernández, Alejandra
A1 Pérez-García, Alejandro
A1 Fernández-Ortuño, Dolores
K1 Interacciones ARN
K1 Plantas transgénicas
K1 Microbiología agrícola
K1 Hongos fitopatógenos
AB Powdery mildew and rust fungi are major agricultural problems affecting many economically important crops and causing significant yield losses. These fungi are obligate biotrophic parasites that are completely dependent on their hosts for growth and reproduction. Biotrophy in these fungi is determined by the presence of haustoria, specialized fungal cells that are responsible for nutrient uptake and molecular dialogue with the host, a fact that undoubtedly complicates their study under laboratory conditions, especially in terms of genetic manipulation. RNA interference (RNAi) is the biological process of suppressing the expression of a target gene through double-stranded RNA that induces mRNA degradation. RNAi technology has revolutionized the study of these obligate biotrophic fungi by enabling the analysis of gene function in these fungal. More importantly, RNAi technology has opened new perspectives for the management of powdery mildew and rust diseases, first through the stable expression of RNAi constructs in transgenic plants and, more recently, through the non-transgenic approach called spray-induced gene silencing (SIGS). In this review, the impact of RNAi technology on the research and management of powdery mildew and rust fungi will be addressed.
PB MDPI
YR 2023
FD 2023-05-02
LK https://hdl.handle.net/10630/27138
UL https://hdl.handle.net/10630/27138
LA eng
NO Padilla-Roji I, Ruiz-Jiménez L, Bakhat N, Vielba-Fernández A, Pérez-García A, Fernández-Ortuño D. RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi. International Journal of Molecular Sciences. 2023; 24(10):9082. https://doi.org/10.3390/ijms24109082
NO Partial funding for open access charge: Universidad de Málaga
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026