RT Conference Proceedings
T1 Whole genome, transcriptome, smallRNAome and methylome profiling during tomato-geminivirus interaction
A1 Romero-Rodríguez, Beatriz
A1 Kriznik, Maja
A1 Morilla, Ian
A1 Petek, Marko
A1 Jiao, Chen
A1 Fei, Zhangjun
A1 Gruden, Kristina
A1 Rodríguez-Bejarano, Eduardo
A1 Castillo-Garriga, Araceli
K1 Virus fitopatógenos
K1 Tomates - Enfermedades y plagas
AB Geminiviruses constitute the largest familyof plant-infecting viruses with small,single- stranded DNA genomes that replicatethrough double-stranded DNAintermediates. Because of their limitedcoding capacity, geminiviruses useplant nuclear machinery to amplify theirgenomes, which are packaged into nucleosomesforming chromatin as multiplecircular minichromosomes. Thus,viral minichromosomes must encounterthe nuclear pathways that regulate hostgene expression and chromatin states.DNA methylation and post-transcriptionalgene silencing play critical rolesin controlling infection of geminivirusesand this pathogen can counteract thesehost defense mechanisms and promoteits infectivity. Tomato Yellow LeafCurl Virus (TYLCV) belongs to the Begomovirusgenus and is transmitted by thewhitefly Bemisia tabaci. With only sevenviral proteins, TYLCV must create a properenvironment for viral replication,transcription, and propagation. Behindthe apparent simplicity of geminiviruseslies a complex network of molecular interactionswith their host and their naturalvector, which induces a wide varietyof transcriptional, post-transcriptionaland chromatin changes in the host. Tobetter understand this virus-host interactionat a genetic and epigenetic levelwe carried out a global approach of theTYLCV-tomato interaction to generateintegrated single-base resolution mapsby Next-Generation Sequencing of thetranscriptome, smallRNAome and methylomeof the pathogen and the host.Total RNA and DNA was extracted fromtomato–infected plants (three biologicalreplicates) and analysed at 2, 7, 14and 21-day post-infection (dpi). Analysisof the changes in host transcription duringthe infection and its correlation with changes in sRNA profiles (microRNA and phasiRNA) and DNA methylation patternswill be presented and discussed.
YR 2022
FD 2022
LK https://hdl.handle.net/10630/24432
UL https://hdl.handle.net/10630/24432
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026