RT Journal Article
T1 Immune response of DNA vaccinated-gilthead seabream (Sparus aurata) against LCDV-Sa infection: relevance of the inflammatory process
A1 Leiva Rebollo, Rocío
A1 Gémez Mata, Juan
A1 Castro-López, María Dolores
A1 Borrego García, Juan José
A1 Labella Vera, Alejandro Manuel
K1 Cultivos - Virus
AB Lymphocystis disease is one of the main viral pathologies affecting culturedgilthead seabream (Sparus aurata) in the Mediterranean region. Recently, wehave developed a DNA vaccine based on the major capsid protein (MCP) of theLymphocystis disease virus 3 (LCDV-Sa). The immune response triggered byeither LCDV-Sa infection or vaccination have been previously studied and seemto be highly related to the modulation of the inflammatory and the IFN response.However, a comprehensive evaluation of immune-related gene expression invaccinated fish after viral infection to identify immunogenes involved in vaccineinduced protection have not been carried out to date. The present study aimedto fulfill this objective by analyzing samples of head-kidney, spleen, intestine, andcaudal fin from fish using an OpenArray® platform containing targets related tothe immune response of gilthead seabream. The results obtained showed anincrease of deregulated genes in the hematopoietic organs between vaccinatedand non-vaccinated fish. However, in the intestine and fin, the results showedthe opposite trend. The global effect of fish vaccination was a significantdecrease (p<0.05) of viral replication in groups of fish previously vaccinated,and the expression of the following immune genes related to viral recognition(tlr9), humoral and cellular response (rag1 and cd48), inflammation (csf1r, elam,il1b, and il6), antiviral response (isg15, mx1, mx2, mx3), cell-mediated cytotoxicity(nccrp1), and apoptosis (prf1). The exclusive modulation of the immune responseprovoked by the vaccination seems to control the progression of the infection inthe experimentally challenged gilthead seabream.
PB Frontiers
YR 2023
FD 2023-06-06
LK https://hdl.handle.net/10630/33083
UL https://hdl.handle.net/10630/33083
LA eng
NO Leiva-Rebollo R, Ge´ mez-Mata J, Castro D, Borrego JJ and Labella AM (2023) Immune response of DNA vaccinated-gilthead seabream (Sparus aurata) against LCDV-Sa infection: relevance of the inflammatory process. Front. Immunol. 14:1209926. doi: 10.3389/fimmu.2023.1209926
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026