RT Conference Proceedings T1 Transcriptome analysis of Neofusicoccum luteum during avocado branch and fruit infection A1 Guirado-Manzano, Lucía A1 Moreno-Pérez, Ana A1 Fernández-Pozo, Noé A1 Cazorla-López, Francisco Manuel A1 Fernández-Ortuño, Dolores A1 Arrebola-Díez, Eva María A1 Pliego-Prieto, Clara K1 Aguacate - Plagas AB The most important aerial diseases affecting avocado orchards on theAndalusian coast (Spain) are caused by species of fungi belonging tothe Botryosphaeriaceae family, one of the most common being Neofusicoccumluteum. The symptoms produced by this fungus are branchdieback and fruit rot. Currently, it is essential to develop strategies tocontrol this disease. In this line, knowledge of the infection mechanismsof the pathogen is considered an essential objective. Therefore,a transcriptomic study was carried out by RNAseq of N. luteum growingon avocado branch and fruit in comparison with its in vitro growthin PDA medium. Transcriptome analysis revealed a total of 903 and1271 genes significantly downregulated (- 2 > fold change > 2) duringgrowth on branch and fruit compared to growth on PDA, respectively.Among the genes overexpressed in the N. luteum/branch/fruit interaction,genes related to mycotoxin production, wall degradation, detoxificationof harmful compounds, protein degradation and candidate effectorproteins were identified, three of which showed 100% probability(Effector P3) and apoplastic localization. The analysis of N. luteumtranscriptome during the infection process will be a very useful tool tobetter understand the biology and virulence of this emerging pathogenand will help in the development of strategies for its control PB ECFG16 YR 2023 FD 2023-03-13 LK https://hdl.handle.net/10630/26136 UL https://hdl.handle.net/10630/26136 LA eng NO Guirado-Manzano et al. 2023. 16th European Conference on Fungal Genetic, pág. 621 NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. DS RIUMA. Repositorio Institucional de la Universidad de Málaga RD 3 mar 2026