RT Conference Proceedings T1 Resistance to SDHI fungicides in Botrytis cinerea from strawberry fields in Spain A1 Fernández-Ortuño, Dolores A1 Vielba-Fernández, Alejandra A1 Torés, Juan Antonio A1 Pérez-García, Alejandro A1 De-Vicente-Moreno, Antonio K1 Fungicidas AB Gray mold, caused by Botrytis cinerea Pers., is one of the most economically importantdiseases of strawberries and its control involves the application of fungicides throughout thestrawberry growing season. Succinate dehydrogenase inhibitors (SDHIs) constitute a novel class offungicides representing new alternatives for strawberry growers. In the present study, B.cinerea isolates were used to determine the effective concentration that reduces mycelialgrowth by 50% (EC50) and to obtain discriminatory doses to monitor SDHI fungicides over thecourse of three-year monitoring period. The overall frequencies of resistance to the SDHIfungicides boscalid, fluopyram, fluxapyroxad and penthiopyrad were 56.9, 6.9, 12.9, and24.6%, respectively. Four SDHI resistance patterns were observed in our population.Patterns I (resistance to boscalid) and II (resistance to boscalid and penthiopyrad) wereassociated with the amino acid substitutions H272R/Y; pattern III (resistance to boscalid,fluxapyroxad, and penthiopyrad) was associated only with the H272Y mutation; and finally,pattern IV (resistance to boscalid, fluopyram, fluxapyroxad and penthiopyrad) was associated withthe N230I allele in the SdhB subunit. For gray mold management, it is suggested that thesimultaneous use of boscalid and penthiopyrad should be limited to one application per season.The use of fluxapyroxad and fluopyram could be used as valid SDHI alternatives for our strawberrygrowers, but they should be applied with caution. YR 2018 FD 2018-10-17 LK https://hdl.handle.net/10630/16655 UL https://hdl.handle.net/10630/16655 LA eng NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. DS RIUMA. Repositorio Institucional de la Universidad de Málaga RD 4 mar 2026