RT Conference Proceedings
T1 Strawberry GRN forever: insights into the transcriptional regulatory network controlling strawberry fruit ripening and quality
A1 Martín-Pizarro, Carmen
A1 Sánchez-Gómez, Carlos
A1 Meco, Victoriano
A1 Urrutia, María
A1 Pillet, Jeremy
A1 Lucas Reina, Eva
A1 Trapero-Mozos, Almudena
A1 Vallarino Castro, José G.
A1 De Luis Balaguer, María Angels
A1 Pérez-Sancho, Jessica
A1 Bayer, Emmanuelle M.
A1 Franco-Zorrila, José Manuel
A1 Osorio-Algar, Sonia
A1 Sozzani, Rosangela
A1 Botella-Mesa, Miguel Ángel
A1 Valpuesta-Fernández, Victoriano
A1 Posé-Padilla, David
K1 TF
K1 Frutas-Calidad-Investigación genética
K1 Strawberry ananassa-Mejora genética
K1 Fresas - Mapa genético
K1 Cultivos - Ingeniería genética
K1 Transcripción genética
K1 Factores de transcripción
K1 Frutas - Sabor y olor
AB Ripening is a critical step for the development of flavor quality in fruits. This character has significantly declined in many fleshy fruits over recent decades. This is particularly significant in strawberry (Fragaria × ananassa), where current cultivars are derived from a narrow germplasm collection. Improving fruit quality requires two important breakthroughs: 1) a precise understanding of the fruit ripening process that will allow the targeting of relevant genes, and 2) the identification of novel alleles responsible for fruit quality traits.In our project, we aim at the identification and characterization of key transcription factors (TF) involved in fruit ripening regulation and their target genes, in order to infer the Gene Regulatory Network controlling this process. Among them, we have identified two TFs belonging to the NAC (FaRIF) and the BLH9 (FaRPL) family. Functional analyses establishing stable silencing and overexpression lines support that both TFs play a critical role in the regulation of fruit ripening and development. Furthermore, using a stage- and tissue-specific transcriptome analysis, we have identified TFs specifically expressed in the external layer of ripe receptacles of F. vesca fruits, which are involved in the regulation of wax and cuticle formation.Finally, we have implemented the use of the genome-editing tool CRISPR/Cas9 in the cultivated strawberry, which we expect to open opportunities for engineering this species to improve traits of economic importance.
YR 2022
FD 2022-07-01
LK https://hdl.handle.net/10630/24291
UL https://hdl.handle.net/10630/24291
LA eng
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026