2026-06-06T05:02:35Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/142562026-02-03T12:07:18Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Vida Hinojosa, Carmen María
author
Tienda Serrano, Sandra
author
De-Vicente-Moreno, Antonio
author
Cazorla-López, Francisco Manuel
author
Gutiérrez Barranquero, José Antonio
2017-07-18T11:23:49Z
2017-07-18T11:23:49Z
2017-07-18
http://hdl.handle.net/10630/14256
http://orcid.org/0000-0003-0798-0964
Backgrounds
This study focused on the characterization and selection of bacterial strains obtained from a suppressive soil displaying antifungal activity against the soilborne phytopathogenic fungi Rosellinia necatrix. Bacterial profile from this suppressive soil were first obtained by 16S rRNA gene sequencing, revealing a significant increase in the bacterial class Gammaproteobacteria, especially in some antagonistic representatives of Pseudomonas spp.
Objectives
To obtain and characterize a collection of 246 bacterial isolates obtained from this suppressive soil, in order to identify new strains with antifungal activity against fungal phytopathogens.
Methods
To obtain the bacterial collection, we performed an isolation on a selective medium for Pseudomonas-like microorganisms. Further characterization tests were used in order to analyse the bacterial collection, including identification of the general metabolic profile of glucose, the profiling of antifungals produced, including both the putative production of antifungal compounds and lytic exoenzymes, and the evaluation of traits related with beneficial effects on plants.
Conclusions
A final selection of representative strains resulted in antifungal isolates belonging to the genus Pseudomonas, but also some representatives of the genera Serratia and Stenotrophomonas. These selected strains were tested for plant protection by an in vivo experiment using avocado and wheat plants challenged by the pathogen R. necatrix, showing all of them an antifungal ability and plant disease protection.
Pseudomonas-like strains isolated from suppressive soils constitute an excellent source for novel microbial biocontrol agents against soilborne fungal pathogens.
This work was supported by grant AGL2014-52518-C2-1-R. Carmen Vida and Sandra Tienda are supported by a PhD fellowship from the FPI program of the Spanish Government.
eng
by-nc-nd
Hongos fitopatógenos
Isolation, characterization and selection of bacterial isolates from a suppressive soil with beneficial traits to plants
conference output
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URL
https://riuma.uma.es/bitstreams/3be4a5ae-cd22-4c55-a639-2bad5f909496/download
File
MD5
64a0f60707dc0e565cf9e117a6b3e052
181281
application/pdf
Vida et al FEMS 17.pdf