2026-05-27T05:35:43Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/143292026-02-03T12:25:48Zcom_10630_2254col_10630_37959

LPA1/3 receptor antagonist KI16425 as a novel treatment for the neurobehavioural effects of the ethanol Ladrón de Guevara-Miranda, David Sánchez-Marín, Laura Alén, Francisco Moreno-Fernández, Román D. García-Fernández, María Inmaculada Gil Rodríguez, Sara Gavito, Ana L. García-Marchena, Nuria Pedraza-Benítez, María del Carmen Pavón-Morón, Francisco Javier Rodriguez-de-Fonseca, Fernando Santín-Núñez, Luis Javier Serrano, Antonia Castilla-Ortega, María Estela Hipocampo (Cerebro) Aims. The lysophosphatidic acid (LPA) is an ubiquitous lysophospholipid that acts through G-protein coupled receptors (LPA1-6), and it is involved in the modulation of emotional and motivational behaviors. Recent literature suggests a relevant role of the LPA signaling system in alcoholism, specially through the LPA1 receptor. This work aims to elucidate whether systemic LPA1/3 receptor blockade with ki16425 would modulate ethanol effects on the brain and behavior. Methods. This study consisted of four experiments assessing the effect of intraperitoneal ki16425 administration (20 mg/kg) on ethanol-related behaviors. Male Wistar rats or mice (Swiss, C57BL/6J or hybrid C57BL/6J×129X1/SvJ background) were employed in various procedures: I) oral ethanol selfadministration; II) loss of righting reflex; III) ethanol-induced conditioned place preference (CPP) and IV) ethanol-withdrawal behavioral symptoms (by assessing nest building, physical signs and spatial working memory). Immunohistochemistry was carried out in order to evaluate basal neuronal activity (c-Fos) in the medial prefrontal cortex (mPFC) and in the hippocampus, as well as adult hippocampal neurogenesis (AHN) using proliferating cell nuclear antigen (PCNA) and doublecortin (DCX) markers. Results. Systemic Ki16425 administration reduced oral self-administration of ethanol in previously trained rats. Likewise, ki16425 pretreatment in mice attenuated the sedation induced by ethanol, blocked ethanol rewarding effect in a CPP paradigm and reduced behavioral symptoms induced by ethanol withdrawal. Immunohistochemistry revealed a protective effect of ki16425 against ethanol actions on basal neuronal activity in the mPFC and on AHN. Conclusions. Our results suggest a potential usefulness of systemic LPA1/3 receptors antagonists as a novel treatment for alcohol-related disorders. 2017-07-24T07:02:59Z 2017-07-24T07:02:59Z 2017 2017-07-24 conference output http://hdl.handle.net/10630/14329 eng II Congreso Internacional de Psicobiologia Avila. España 19 de Julio de 2017 open access by-nc-nd