2026-05-30T04:49:57Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/145362026-02-03T11:59:16Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Pintado Calvillo, Adrián
author
Aragón Cortés, Isabel María
author
Pérez-Martínez, Isabel
author
Pliego-Prieto, Clara
author
Crespo-Gómez, José Ignacio
author
De-Vicente-Moreno, Antonio
author
Cazorla-López, Francisco Manuel
author
Ramos-Rodríguez, Cayo Juan
2017-09-26T11:45:12Z
2017-09-26T11:45:12Z
2017-09-26
http://hdl.handle.net/10630/14536
Pseudomonas sp. UMAF110, isolated from rhizosphere soil in Spain, display in vitro antagonism towards the pythopathogenic fungus Rosellinia necatrix and is able grow in fungal exudates (BM-RE medium). A transposon mutant library of this strain was constructed and several mutants were selected by their reduced competitiveness in BM-RE medium. Pseudomonas sp. UMAF110-G3, which contains the transposon into a gene encoding a putative REC/PAS/GGDEF/EAL protein, was selected for further characterization. Blastn searches using the sequence of the gene interrupted by the transposon in UMAF110-G3, here called cmpA (c-di-GMP Metabolizing Protein), yielded a single positive hit (98% cover, 78% identity) with a gene from a terpene-degrading Pseudomonas sp. strain isolated from soil. Context analysis of the cmpA gene in Pseudomonas sp. UMAF110 showed that this gene is located downstream from several genes involved in flagellar motility/chemotaxis. RT-PCR experiments further confirmed that cmpA form a transcriptional unit with the che gene cluster. Expression analysis of cmpA by qRT-PCR clearly showed upregulation of this gene after transfer of Pseudomonas sp. UMAF110 cells to BM-RE medium, suggesting a role for this operon in response to fungal exudates. Deletion of cmpA in Pseudomonas sp. UMAF110 did not affect the ability of the strain to form biofilms under the conditions tested. However, overexpression of wild type CmpA in Pseudomonas putida KT2440 negatively regulated biofilm formation in this strain. Together, these results suggest that CmpA could be involved in signal transduction pathways regulating flagellar motility/chemotaxis in response to fungal exudates.
spa
by-nc-nd
Pseudomonas
Response to fungal exudates of the rhizosphere isolate Pseudomonas sp. UMAF110 involves a GGDEF/EAL domain-containing protein
conference output
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URL
https://riuma.uma.es/bitstreams/d2cff1df-7cd7-498d-9629-21b866f1fbce/download
File
MD5
9bc2474f3627c5d34442ae7d82a070e2
105296
application/pdf
Pseudomonas Astract_Adrián.pdf