2026-05-30T05:34:09Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/145392026-02-03T12:19:21Zcom_10630_2254col_10630_37959

Contribution of the metabolism of phenolic compounds to the virulence of Pseudomonas savastanoi in woody hosts Caballo-Ponce, Eloy Wittich, Regina Michaela van Dillewijn, Pieter Ramos-Rodríguez, Cayo Juan Pseudomonas Most strains of the Pseudomonas syringae complex isolated from the trunks of woody plants and belonging to phylogroups (PGs) 1 and 3 share a genomic region, named WHOP (from woody host and Pseudomonas), which is partially conserved in some PG2 strains. Annotation of the 14 ORFs encoded within this region, which is absent in the genomes of P. syringae strains infecting herbaceous hosts, yielded functions possibly involved in the metabolism of phenolic compounds. The genetic organization of this region was analyzed by RT-PCR in Pseudomonas savastanoi pv. savastanoi (Psv), the causative agent of olive knot disease. The WHOP region is organized in four operons (antABC, catBCA, ipoABC and dhoAB) and three genes transcribed independently (antR, benR and PSA3335_3206). HPLC analyses confirmed that the antABC and catBCA operons mediate the catabolism of anthranilate and catechol, respectively, through the β-ketoadipate pathway. In addition, oxygenase activity on aromatic compounds, tested as the conversion of indole into indigo, was assigned to the ipoABC operon. Pathogenicity tests revealed that deletion of antABC, catBCA or ipoABC in Psv caused reduced symptoms in woody olive plants, while such phenotype was not observed in young micropropagated (non-woody) plants. Similarly, the catBCA and dhoAB operons and the PSA3335_3206 gene (encoding a putative aerotaxis receptor) are also required for full bacterial fitness exclusively in woody olive plants. At present, we are addressing the role in virulence of the orphan benR gene encoded in the WHOP region. 2017-09-26T12:20:56Z 2017-09-26T12:20:56Z 2017 2017-09-26 conference output http://hdl.handle.net/10630/14539 http://orcid.org/0000-0003-0501-3321 eng 16th International Conference on Pseudomonas Liverpool (Reino unido) 5 de septiembre de 2017 open access by-nc-nd