2026-06-06T01:52:01Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/149852026-02-03T11:54:43Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Piedra-Aguilera, Álvaro
author
Jiao, Chen
author
Esteve-Codina, Anna
author
Abad, Marc
author
Pérez-Luna, Ana Isabel
author
Villanueva-Montiel, Francisco
author
Díaz-Pendón, Juan Antonio
author
Fei, Zhangjun
author
Rodríguez-Bejarano, Eduardo
author
Castillo-Garriga, Araceli
2018-01-08T10:49:48Z
2018-01-08T10:49:48Z
2018-01-08
https://hdl.handle.net/10630/14985
Geminiviruses constitute the largest family of plant-infecting viruses with small, single- stranded DNA genomes that replicate through double-stranded DNA intermediates. Because of their limited coding capacity, geminiviruses use plant nuclear machiney to amplify their genomes, which are packaged into nucleosomes forming chromatin as multiple circular minichromosomes. Thus, viral minichromosomes must encounter the nuclear pathways that regulate host gene expression and chromatin states. DNA methylation and post-transcriptional gene silencing play critical roles in controlling infection of geminiviruses and this pathogen can counteract these host defense mechanisms and promote its infectivity.
To better understand this virus-host interaction at a genetic and epigenetic level we have analysed the transcriptome, sRNA profile and methylome of tomato plants infected with the geminivirus, TYLCV (Tomato yellow leaf curl virus). Total RNA and DNA was extracted from tomato–infected plants (three biological replicates) and analysed at 2, 7, 14 and 21 days pots-infection (dpi). Analysis of the changes in host transcription during the infection and its correlation with changes in sRNA profiles and DNA methylation will be discussed/shown.
This work represents the first comprehensive study of the genetic and epigenetics interactions established during a geminivirus infection in its natural host.
eng
Virus fitopatógenos
Intregrated transcriptome, methylome and smallRNA profile analysis of a geminivirus infection
conference output
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URL
https://riuma.uma.es/bitstreams/200a7bdd-1fc2-4ebc-88aa-faf349a6f181/download
File
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7c416f7ca76632acc720265dc8da8b9e
29881
application/pdf
abstract_A.G.CAstillo.pdf