2026-05-28T19:38:11Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/161472026-02-03T12:25:11Zcom_10630_2254col_10630_37959

Tuning pKa in new molecules based on indolines for two-photon absorption bioimaging applications Benítez-Martín, Carlos Nájera-Albendín, Francisco Pérez-de-Inestrosa-Villatoro, Ezequiel Química orgánica - Congresos Fluorescence-based biosensors have become essential tools for modern biology, allowing real-time monitoring of biological processes within living cells. The differences between pH play a significant role in multiple biological process such as proliferation, apoptosis, endocytic process and defense. Therefore, intracellular fluorescent pH probes comprise one of the most widely used families of biosensors in microscopy. In this work, we present the design, synthesis and characterization of new one and two photon pH activatable fluorescent probes based on naphthalene-indoline derivatives with an off-on response to different pH environments. These compounds are easy to achieve with good yields and have good photophysical properties. Simple modifications in the structure of these compounds allow tuning their pKa easily and cover a width range of pH in their applications. The preliminary results obtained with these fluorescent probes indicate that they have promising properties to be use in bioimaging applications in living cells. 2018-07-06T06:46:20Z 2018-07-06T06:46:20Z 2018 2018-06-20 conference output https://hdl.handle.net/10630/16147 eng XXVII Biennial Meeting in Organic Chemistry Santiago de Compostela (España) junio de 2018 open access Real Sociedad Española de Química