2026-05-27T05:29:05Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/161982026-02-03T11:49:40Zcom_10630_2254col_10630_37959

Martín-Pizarro, Carmen Meco, Victoriano Vallarino Castro, José G. Pillet, Jeremy Casañal, Ana Merchante-Berg, Catharina Giovannoni, James J. Osorio-Algar, Sonia Botella-Mesa, Miguel Ángel Posé-Padilla, David Valpuesta-Fernández, Victoriano 2018-07-12T09:06:06Z 2018-07-12T09:06:06Z 2018-07-12 https://hdl.handle.net/10630/16198 Strawberry is becoming a model for studying the molecular mechanism of ripening in non-climacteric fruits. However, a limited number of transcriptional regulators of this process have been identified so far. In this study, we have identified and characterized a gene encoding for a NAC transcription factor (TF), named as FaRIF (Ripening Inducing Factor). FaRIF expression presents a fruit-specific pattern, which is upregulated during ripening. In order to functionally characterize this TF, we have generated silencing (35S::RIF-RNAi) and overexpressing (35S::RIF-GFP) stable transgenic lines. While the RNAi lines showed an apparent delay of fruit ripening, the overexpressing lines displayed an acceleration of this process. Transcriptomic analysis, by RNA-seq, of the silenced lines showed a significantly altered expression of genes involved in the flavonoids pathway, as well as genes of the metabolism of the main sugars of the fruit. Metabolomics analysis confirmed these changes in the transgenic fruits. Both, transcriptomic and metabolomics data, were in agreement with the general phenotype observed in the fruits of the FaRIF-silenced lines. All together, our results support a main role of FaRIF in the control of relevant ripening-associated processes in strawberry fruit. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Fresa FaRIF Transcription Factor Plays a Key Role in the Regulation of Fruit Ripening in the Cultivated Strawberry Fragaria x ananassa conference output