2026-06-06T17:53:20Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/162042026-02-03T12:15:33Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Amorim-Silva, Vitor
author
Esteban del Valle, Alicia
author
Castillo-Garriga, Araceli
author
Rosado Rey, Abel
author
Vanneste, Steffen
author
Perea, Carlos
author
Salinas, Julio
author
Botella-Mesa, Miguel Ángel
author
Ruiz-López, Noemí
author
Pérez-Sancho, Jessica
author
Schapire, Arnaldo
author
Osorio-Algar, Sonia
author
Willmitzer, Lothar
2018-07-12T09:43:41Z
2018-07-12T09:43:41Z
2018-07-12
https://hdl.handle.net/10630/16204
As sessile organisms, plants must cope with abiotic stress such as soil salinity, drought, and extreme temperatures. This stress signal can activate a phospholipase C (PLC), which hydrolyses PIP2 to generate IP3 and diacylglycerol (DAG). ER-PM contact sites are conserved structures defined as regions of the endoplasmic reticulum (ER) that tightly associate with the plasma membrane (PM). Our recent data suggest that the constitutively expressed Arabidopsis Synaptotagmin 1 (SYT1) and the cold-induced homolog SYT3 are proteins located in these ER-PM contact sites that are essential for freezing tolerance. Additionally, like mammalian Extended Synaptotagmins, membrane tethering is mediated by C2-domains which interact with acidic phospholipids (enhanced by Ca2+). Our experiments of depletion of PM PI(4)P triggers loss of SYT1 and SYT3 at ER-PM CS. Moreover, our analysis in SYT1 and SYT3 proteins predicted a SMP domain like the recently crystalized E-SYT2 which exhibits a hydrophobic groove capable of harbouring phospholipids, suggesting that SYT1 and SYT3 mediate lipid exchange between the ER and the PM. This idea is supported by the over-accumulation of saturated DAG found in SYT1 after a high-resolution lipidome analysis. Additionally, we have identified DGK2 (diacylglycerol kinase 2) as an interactor of SYT1. In summary, our recent studies suggest that SYT1 and SYT3 are ER-PM tether components responsible for the elimination of excess DAG from the PM after its acute generation by PLC in cold conditions.
eng
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Biología molecular - Congresos
Arabidopsis Synaptotagmins 1 and 3 are involved in lipid homeostasis at ER-PM contact sites under cold stress
conference output
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URL
https://riuma.uma.es/bitstreams/2d86bf08-5d62-4e14-98e9-01eb16de7d6a/download
File
MD5
daf82739fda892152983a00e71e7607e
26767
application/pdf
NoemiRuiz_abstract-1.pdf