2026-05-28T13:21:33Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/176342026-02-03T11:59:15Zcom_10630_2254col_10630_37959
CRISPR/Cas9-mediated editing of the TM6 MADS-box gene in the octoploid strawberry (Fragaria x ananassa)
Martín-Pizarro, Carmen
Posé-Padilla, David
Ingeniería genética vegetal
The B-class of MADS-box transcription factors has been studied in many plant species, but
remain functionally uncharacterized in the Rosaceae family. APETALA3 (AP3), a member of this
class, controls the identity of petals and stamens in Arabidopsis thaliana. In this work, we identified
two members of the AP3 lineage in the cultivated strawberry (Fragaria × ananassa): FaAP3
and FaTM6. Interestingly, FaTM6, and not FaAP3, shows an expression pattern equivalent to that
of AP3 in Arabidopsis. Genome editing using Cluster Regularly Interspaced Short Palindromic
Repeats (CRISPR)/Cas9 system is becoming a robust tool for targeted and stable mutagenesis
of DNA. However, whether it can be efficiently used in an octoploid species such as F. × ananassa
is not known. In our study, we report the application of the CRISPR/Cas9 in F. × ananassa to
characterize the function of FaTM6 in flower development. An exhaustive analysis by high-throughput
sequencing of the FaTM6 locus spanning the target sites showed a high efficiency genome
editing already in the T0 generation. The phenotypic characterization of the mutant lines
indicates that FaTM6 plays a key role in petal and especially in anther development in strawberry.
Our results validate the CRISPR/Cas9 strategy for gene functional analysis in an octoploid
species such as F. × ananassa, and offer new opportunities for engineering strawberry to improve
traits of interest in breeding programs.
2019-05-10T07:40:55Z
2019-05-10T07:40:55Z
2019-05-10T07:40:55Z
2019-05-10
conference output
https://hdl.handle.net/10630/17634
eng
At the Forefront of Plant Research 2019
Barcelona
6-8 Mayo 2019
http://creativecommons.org/licenses/by-nc-nd/4.0/
open access
Attribution-NonCommercial-NoDerivatives 4.0 Internacional