2026-05-27T05:24:05Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/177802026-02-03T12:05:35Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Luna, Ana P.
author
Sabarit Penalosa, Blanca
author
Arroyo-Mateos, Manuel
author
Maio, Francesca
author
Sánchez-Durán, Miguel A.
author
Rosas-Díaz, Tábata Victoria
author
Prins, Marcel
author
Ruiz-Albert, Francisco Javier
author
Burg, Harrold A. van den
author
Rodríguez-Bejarano, Eduardo
2019-06-07T10:22:26Z
2019-06-07T10:22:26Z
2019-06-07
https://hdl.handle.net/10630/17780
Geminiviruses are plant viruses with circular, single-stranded DNA (ssDNA) genomes that infect a broad range of plants causing substantial crop diseases worldwide. They replicate in nuclei of infected cells by using host DNA replication machinery and an essential protein encoded in their genome designated Rep (replication-associated protein). This multifunctional protein induces the accumulation of the host factors involved in replication and it is capable of interacting with a lot of plant proteins including PCNA (Proliferating Cell Nuclear Antigen), a processivity factor that coordinates a wide range of processes involved in maintenance, duplication and transmission of the genome, and the sumoylation enzyme that conjugates SUMO to target proteins (SUMO-conjugating enzyme- SCE). PCNA modification by SUMO, and also ubiquitin, has long been known to be of key importance for determining how DNA damage is processed by the replisome and for maintenance of overall genome integrity. In yeast, PCNA sumoylation has been associated to DNA repair involving homologous recombination (HR). Previously, we reported that Rep ectopic expression does not result in broad changes in the sumoylation pattern of plant cells, but it modifies the sumoylation state of selected host proteins. In this work, we show, using a reconstituted sumoylation system in Escherichia coli, that tomato PCNA is sumoylated at two residues, K254 and K164, and that co-expression of the Rep protein suppresses PCNA sumoylation at these lysines. Finally, we confirm that PCNA is sumoylated and that Rep also interferes with PCNA sumoylation in planta.
eng
Plantas -- Investigación
Congresos y conferencias
Geminivirus replication protein decreases PCNA sumoylation at two acceptor sites
conference output
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URL
https://riuma.uma.es/bitstreams/4cfd529d-091d-4893-8d2b-696d7f8f6c7a/download
File
MD5
f11c49eabd2710eee02a347907381fd5
1077289
application/pdf
Poster_CRAG2019_APL.pdf