2026-06-02T03:50:54Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/183172026-02-03T12:12:27Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
López-González, Manuel Víctor
author
González-García, Marta
author
Carrillo-Franco, Laura
author
Carrillo-Franco, Cristina
author
Díaz-Casares, Amelia
author
Barbancho-Fernández, Miguel Ángel
author
Dawid-Milner, Marc Stefan
2019-09-11T12:07:12Z
2019-09-11T12:07:12Z
2019-09-11
https://hdl.handle.net/10630/18317
Background: Stimulation of the dorsomedial hypothalamic nucleus and perifornical area (DMH-PeF) in rats evokes a cardiorespiratory response characterised by an increase in blood pressure, heart rate and respiratory frequency. In previous studies (Díaz-Casares et al., 2012; López-González et al., 2018) we have shown a functional interaction between DMH-PeF and pontine structures (A5 region and Parabrachial Complex). We have also shown that rostral and ventral pontine structures are involved in the changes of laryngeal caliber (Lara et al., 2002). The aim of this study was to characterize the relations between hypothalamic and pontine neuronal circuits involved in laryngeal activity and its effect on vocalization.
Methods: Experimental studies were carried out with non-inbred male rats (n=7). Animals were anesthetized with sodium pentobarbitone (60 mg/kg i.p., initial dose, supplemented 2 mg/ kg, i.v., as necessary). A double tracheal cannulation (upwards in direction of the glottis for the “glottis isolated in situ” technique) was done. Subglottic pressure was recorded with an aneroid transducer by passing a stream of humidified medical air upwards through the larynx at a constant rate of 30-70 ml/min with a thermal mass digital air flow meter controller. Electrical stimulation of the DMH-PeF using concentric bipolar electrodes (1 ms pulses, 20-40 µA, 100 Hz for 5 s) was performed. Respiratory flow, pleural pressure, blood pressure, heart rate and ECG activity were also recorded.
Results: DMH-PeF stimulation evoked a decrease of laryngeal resistance (subglottal pressure) (p<0,001) accompanied with an inspiratory facilitatory response consisted of an increase in respiratory rate (p<0,001), together with a pressor (p<0,001) and a tachycardic response (p<0,001).
Conclusions: The results of our study contribute with new data on the role of the DMH-PeF in the mechanisms controlling subglottic pressure and laryngeal activity.
eng
Respiración
Laryngeal effects of stimulation of the dorsomedial hypothalamic nucleus and perifornical area in spontaneously breathing anaesthetized rats
conference output
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URL
https://riuma.uma.es/bitstreams/b9cb30bf-f796-48ce-b702-a3003cc7db8c/download
File
MD5
270ba6444afe63458fb34786d973f28f
43414
application/pdf
LARINGEAL EFFECTS HDA.pdf