2026-05-29T23:32:44Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/186412026-02-03T12:07:03Zcom_10630_2254col_10630_37959
“Off-on” two-photon indolenine probes for pH bioimaging
Benítez-Martín, Carlos
Guadix-Domínguez, Juan Antonio
Pearson, John R.
Nájera-Albendín, Francisco
Pérez-Pomares, José María
Pérez-de-Inestrosa-Villatoro, Ezequiel
Biodetectores
Microscopía de fluorescencia
Sondas fluorescentes
Iones hidrógeno - Concentración
Fluorescence-based biosensors have become an indispensable tool in modern biology. These molecules can be used to monitor processes inside living cells and in real-time. The control of the pH is crucial in multiple biological process such as proliferation, apoptosis and defense. Therefore, the use of such pH probes are widespread in fluorescence microscopy.
Recently, we have described a biosensor with excellent photophysical and suitable two-photon absorption (TPA) properties. This sensor allows the detection of hydroxyl radicals inside lysosomes. Based on this scaffold, we have designed, synthesized and characterized a new TPA fluorescent probe with an “off-on” response to different pH environments. These naphthalene-indolenine derivatives have a high synthetic versatility through affordable and efficient synthesis. The preliminary results suggest that this sensor has promising properties for detecting pH changes inside living cells.
2019-10-25T07:26:09Z
2019-10-25T07:26:09Z
2019-10-25T07:26:09Z
2019-10-25
conference output
https://hdl.handle.net/10630/18641
eng
IX Reunión de Química Orgánica del Mediterráneo
Murcia (España)
23/10/2019
open access