2026-05-30T20:25:01Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/208222026-02-03T12:21:04Zcom_10630_2254col_10630_37959

00925njm 22002777a 4500 dc Urrutia, María author Meco, Victoriano author Rambla, José Luis author Toivainen, Tuomas author Pillet, Jeremy author Trapero-Mozos, Almudena author Martín-Pizarro, Carmen author Sánchez-Gómez, Carlos author Salas, Joaquín author Granell, Antonio author Hytönen, Timo author Posé-Padilla, David author 2021-01-22 Woodland strawberry (Fragaria vesca, 2x) is the diploid closest ancestor of the cultivated strawberry (Fragaria x annassa, 8x) and the model species for genetic studies in the Fragaria genus. It is naturally distributed all across Europe and it is appreciated for their delicate aroma and flavor. Methylketones are compounds with demonstrated insect repellent effects. Its synthesis, reported to take place in glandular trichomes of wild tomato (Solanum habrochaites), is mediated by a thioesterase (ShMKS2) and a decarboxylase (ShMKS1) (Ben Israel et al. 2009; Yu et al. 2010). A natural population of 199 accessions of F. vesca that represents the European diversity was resequenced (>1.8M SNPs) and characterized for the accumulation of methylketones and their derived alcohols. By genome wide association analysis (GWAS), we have detected a stable series of polymorphisms associated to the accumulation of C7, C9 and C11 methylketones and their respective alcohols. Three different haplotypes in the candidate region with different patterns of methylketones were found, suggesting differences in enzymatic activity and/or substrate affinity. The candidate region includes two genes annotated as thioesterases that are homolog to ShMKS2, FvMKS2A, FvMKS2B. Functional validation of both genes has revealed methyl ketones synthesis ability in transient expression in Nicotiana benthamiana. The function and specificity of these candidate genes is currently under study through several approaches, including transient expression in different hosts and enzymatic function characterization. https://hdl.handle.net/10630/20822 Fresas Olores Identification of candidate genes for methylketones content in a collection of european woodland strawberry