2026-06-03T11:43:33Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/208272026-02-03T12:32:34Zcom_10630_2254col_10630_37959

Effects of Galanin N-Terminal fragment (1-15) in the anhedonic behavioural tests in rats. García-Cantero, Noelia Flores-Burgess, Antonio Gago-Calderón, Belén García-Durán, Laura Alén, Francisco Orio, Laura Narváez-Bueno, José Ángel Fuxe, Kjell Santín-Núñez, Luis Javier Díaz-Cabiale, Zaida Millón-Peñuela, Carmelo Biomedicina -- Investigación The Galanin N-terminal fragment (1-15)[GAL(1-15)] induces depressant- and anxiogenic-like actions in behavioural tests in rats. Since anhedonia is a core feature of depression, we have analyzed GAL(1-15) actions in anhedonic-like behaviour tests: saccharin Self-administration, Sucrose Preference test (SPT), Novelty suppressed feeding (NSF) and Female urine sniffing test (FUST). Three sets of experiments were conducted in the saccharin Self-administration. First, a dose-response curve of GAL(1-15) 1nmol, 3nmol or vehicle was performed. We have also compared the effects in the number of saccharine reinforcements of GAL 3nmol and GAL(1-15) 3nmol. In the last experiments, rats received GAL(1-15) 3nmol and the GALR2 antagonist M871 3nmol. In SPT, NSF and FUST we have analyzed the effects of GAL(1-15) 3nmol in the sucrose intake and preference, the latency of the first feeding episode and the female urine sniffing duration respectively. GAL(1-15)3nmol significantly decreased the number of reinforcement of saccharin self-administer (p<0.01), while 1nmol lacked effect. GAL(1-15) also significantly reduced the number of reinforcement (p<0.01) compared with GAL. The GALR2 antagonist significantly blocked (p<0.05) the decrease in the number of saccharin reinforcements induced by GAL(1-15). The administration of GAL(1-15) decreased the sucrose intake 8 (p<0.05) and 24 hours (p<0.01) In the SPT, increased the latency of feeding (p<0.001) in the NSF and significantly decreased sniffing duration in the FUST. All these results indicate that GAL(1-15) induces a strong anhedonia-like phenotype in several behavioural tests, confirming an important role of this neuropeptide in anhedonia. 2021-01-22T15:33:12Z 2021-01-22T15:33:12Z 2021-01-22T15:33:12Z 2021-01-22 conference output https://hdl.handle.net/10630/20827 eng FENS Glasgow Julio 2020 open access