2026-05-28T12:15:09Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/213762026-02-03T12:30:00Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Cáceres Palomo, Laura
author
García-León, Juan Antonio
author
Dávila-Cansino, José Carlos
author
Vitorica Ferrández, Javier
author
Gutiérrez-Pérez, Antonia
2021-04-14T12:19:38Z
2021-04-14T12:19:38Z
2021
https://hdl.handle.net/10630/21376
Aims: Alzheimer's disease (AD) is characterized by presenting a complex pathology, not fully resolved yet. This fact, together with the lack of reliable models, has impeded the development of effective therapies. Recently, several studies have shown that functional glial cell defects have a key role in the pathology of AD. However, this glial dysfunction, currently, cannot be correctly modeled using the available animal models, so we hypothesized that cells derived from Alzheimer's patients can serve as a better platform for studying the disease. In this sense, human pluripotent stem cells (hPSC) allow the generation of different types of neural cells, which can be used for disease modeling, identification of new targets and drugs development. Methods: We have a collection of hiPSCs derived from patients with sporadic forms of AD. We have differentiated these cells towards neural lineage to obtain neurons and astrocytes. For the generation of oligodendrocytes (OLs), we have developed a fast and robust protocol to generate mature OLs in just 22 days. Results: We have generated neural precursors from all the lines tested. In the case of OLs, the cells generated resemble primary OLs and can myelinate neurons in vivo and in vitro using a screening compatible platform. This platform is being transferred for the generation of the other glial cells. Conclusions: This methodology can be used to elucidate the pathogenic pathways associated with neurodegeneration and to identify new therapeutic targets susceptible to modulation, contributing to the development of new effective drugs against AD.
spa
Alzheimer, Enfermedad de
Suitability of human-derived cells as a platform for Alzheimer's disease modeling
conference output
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URL
https://riuma.uma.es/bitstreams/2606bfed-999a-4d68-bacd-79426ecee1cc/download
File
MD5
2493f30498cdc66e1dfb3fef79eee36f
194173
application/pdf
Poster abstracts ADPD 2021.pdf