2026-05-29T21:14:54Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/235662026-02-03T11:58:03Zcom_10630_2254col_10630_37959

00925njm 22002777a 4500 dc Martín-Pizarro, Carmen author Vallarino Castro, José G. author Osorio-Algar, Sonia author Meco, Victoriano author Urrutia, María author Merchante-Berg, Catharina author Amaya Saavedra, Iraida author Willmitzer, Lothar author Fernie, Alisdair R author Giovannoni, James J. author Botella-Mesa, Miguel Ángel author Valpuesta-Fernández, Victoriano author Posé-Padilla, David author 2021-09-13 Strawberry is becoming a model for studying the molecular mechanism of ripening in non-climacteric fruits. However, a limited number of transcriptional regulators of this process have been identified so far. In this study, we have identified and characterized a gene encoding for a NAC transcription factor (TF), named as FaRIF (Ripening Inducing Factor). FaRIF expression presents a fruit-specific pattern, which is upregulated during ripening. In order to functionally characterize this TF, we have generated silencing and overexpressing stable transgenic lines. While the RNAi lines showed an apparent delay of fruit ripening, the overexpressing lines displayed an acceleration of this process. Transcriptomic analysis of the silenced lines showed a significantly altered expression of genes involved in development, hormone metabolism, flavonoid pathway, and cell-wall disassembly, being many of these confirmed by phenotypical and metabolomics analysis. Our results support a main role of FaRIF in the control of relevant ripening-associated processes in strawberry fruit. https://hdl.handle.net/10630/23566 Fresas Identification and characterization of the NAC transcription factor FaRIF, a key regulator of strawberry fruit ripening