2026-05-30T01:33:46Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/235742026-02-03T12:19:19Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Urrutia, María
author
Meco, Victoriano
author
Rambla, José L
author
Toivainen, Tuomas
author
Pillet, Jeremy
author
Trapero Mozos, Almudena
author
Martín-Pizarro, Carmen
author
Salas, Joaquín
author
Granell, Antonio
author
Hytönen, Timo
author
Posé-Padilla, David
2022-01-12T07:53:06Z
2022-01-12T07:53:06Z
2021-11-26
https://hdl.handle.net/10630/23574
Woodland strawberry (Fragaria vesca, 2x) is the diploid closest ancestor of the cultivated strawberry (F. x ananassa, 8x) and the model species for genetic studies in the Fragaria genus. It is naturally distributed across Europe and it is appreciated for its delicate aroma and flavor.
Methyl ketones are compounds with demonstrated insect repellent effects (Antonious et al., 2003). Its synthesis, reported to take place in glandular trichomes of wild tomato (Solanum habrochaites), is mediated by a thioesterase (ShMKS2) and a decarboxylase (ShMKS1) (Fridman et al., 2005; Ben-Israel et al., 2009; Yu et al., 2010).
Through genome wide association analysis (GWAS) in a natural collection of European woodland strawberry (199 accessions), we identified a series of polymorphisms linked to the accumulation of C7, C9 and C11 methyl ketones (2-heptanone, 2-nonanone, 2-undecanone), their respective secondary alcohols (2-heptanol, 2-nonanol, 2-undecanol) and their precursors (methyl octanoate,
methyl decanoate, methyl dodecanoate). In addition, the candidate region presented three different haplotypes with different patterns of methyl ketones accumulation, suggesting differences in enzymatic activity and/or substrate
affinity.
The candidate region includes two thioesterases homologues to ShMKS2, FvMKS2A, FvMKS2B. Functional validation of both genes has revealed methyl ketones synthesis ability in transient expression in Nicotiana benthamiana and E. coli. The specificity of these candidate genes is currently under study though several approaches, including transient expression in different hosts and
enzymatic function characterization.
eng
Attribution-NonCommercial-NoDerivatives 4.0 Internacional Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Fresas
Identification of genes responsible of the synthesis of the volatile Methyl Ketone in Woodland Strawberry
conference output
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