2026-05-31T12:34:50Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/242912026-02-03T12:33:58Zcom_10630_2254col_10630_37959

00925njm 22002777a 4500 dc Martín-Pizarro, Carmen author Sánchez-Gómez, Carlos author Meco, Victoriano author Urrutia, María author Pillet, Jeremy author Lucas Reina, Eva author Trapero-Mozos, Almudena author Vallarino Castro, José G. author De Luis Balaguer, María Angels author Pérez-Sancho, Jessica author Bayer, Emmanuelle M. author Franco-Zorrila, José Manuel author Osorio-Algar, Sonia author Sozzani, Rosangela author Botella-Mesa, Miguel Ángel author Valpuesta-Fernández, Victoriano author Posé-Padilla, David author 2022-07-01 Ripening is a critical step for the development of flavor quality in fruits. This character has significantly declined in many fleshy fruits over recent decades. This is particularly significant in strawberry (Fragaria × ananassa), where current cultivars are derived from a narrow germplasm collection. Improving fruit quality requires two important breakthroughs: 1) a precise understanding of the fruit ripening process that will allow the targeting of relevant genes, and 2) the identification of novel alleles responsible for fruit quality traits. In our project, we aim at the identification and characterization of key transcription factors (TF) involved in fruit ripening regulation and their target genes, in order to infer the Gene Regulatory Network controlling this process. Among them, we have identified two TFs belonging to the NAC (FaRIF) and the BLH9 (FaRPL) family. Functional analyses establishing stable silencing and overexpression lines support that both TFs play a critical role in the regulation of fruit ripening and development. Furthermore, using a stage- and tissue-specific transcriptome analysis, we have identified TFs specifically expressed in the external layer of ripe receptacles of F. vesca fruits, which are involved in the regulation of wax and cuticle formation. Finally, we have implemented the use of the genome-editing tool CRISPR/Cas9 in the cultivated strawberry, which we expect to open opportunities for engineering this species to improve traits of economic importance. https://hdl.handle.net/10630/24291 TF Frutas-Calidad-Investigación genética Strawberry ananassa-Mejora genética Fresas - Mapa genético Cultivos - Ingeniería genética Transcripción genética Factores de transcripción Frutas - Sabor y olor Strawberry GRN forever: insights into the transcriptional regulatory network controlling strawberry fruit ripening and quality