2026-05-29T22:12:09Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/244322026-02-03T12:33:50Zcom_10630_2254col_10630_37959

Whole genome, transcriptome, smallRNAome and methylome profiling during tomato-geminivirus interaction Romero-Rodríguez, Beatriz Kriznik, Maja Morilla, Ian Petek, Marko Jiao, Chen Fei, Zhangjun Gruden, Kristina Rodríguez-Bejarano, Eduardo Castillo-Garriga, Araceli Virus fitopatógenos Tomates - Enfermedades y plagas Geminiviruses constitute the largest family of plant-infecting viruses with small, single- stranded DNA genomes that replicate through double-stranded DNA intermediates. Because of their limited coding capacity, geminiviruses use plant nuclear machinery to amplify their genomes, which are packaged into nucleosomes forming chromatin as multiple circular minichromosomes. Thus, viral minichromosomes must encounter the nuclear pathways that regulate host gene expression and chromatin states. DNA methylation and post-transcriptional gene silencing play critical roles in controlling infection of geminiviruses and this pathogen can counteract these host defense mechanisms and promote its infectivity. Tomato Yellow Leaf Curl Virus (TYLCV) belongs to the Begomovirus genus and is transmitted by the whitefly Bemisia tabaci. With only seven viral proteins, TYLCV must create a proper environment for viral replication, transcription, and propagation. Behind the apparent simplicity of geminiviruses lies a complex network of molecular interactions with their host and their natural vector, which induces a wide variety of transcriptional, post-transcriptional and chromatin changes in the host. To better understand this virus-host interaction at a genetic and epigenetic level we carried out a global approach of the TYLCV-tomato interaction to generate integrated single-base resolution maps by Next-Generation Sequencing of the transcriptome, smallRNAome and methylome of the pathogen and the host. Total RNA and DNA was extracted from tomato–infected plants (three biological replicates) and analysed at 2, 7, 14 and 21-day post-infection (dpi). Analysis of the changes in host transcription during the infection and its correlation with changes in sRNA profiles (microRNA and phasiRNA) and DNA methylation patterns will be presented and discussed. 2022-06-20T07:03:20Z 2022-06-20T07:03:20Z 2022-06-20 2022 conference output https://hdl.handle.net/10630/24432 eng III Reunión de la Red Nacional de Virología de Plantas - RENAVIPLANT Murcia, España 25 de mayo de 2022 open access