2026-05-30T02:54:48Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/245212026-02-03T11:14:12Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Ariza Veguillas, Adriana author Mayorga Mayorga, Cristobalina author Bogas Herrera, Gádor author Gaeta, Francesco author Salas, María author Valluzzi, Rocco Luigi author Labella, Marina author Pérez-Sánchez, Natalia Isabel author Caruso, Cristiano author Molina, Ana author Fernández-Duarte, Tahía Diana author Torres-Jaén, María Josefa author Romano, Antonino author 2022-06-23 Diagnosis of type I hypersensitivity reactions (IgE-mediated reactions) to penicillins is based on clinical history, skin tests (STs), and drug provocation tests (DPTs). Among in vitro complementary tests, the fluoro-enzyme immunoassay (FEIA) ImmunoCAP® (Thermo-Fisher, Waltham, MA, USA) is the most widely used commercial method for detecting drug-specific IgE (sIgE). In this study, we aimed to analyze the utility of ImmunoCAP® for detecting sIgE to penicillin G (PG) and amoxicillin (AX) in patients with confirmed penicillin allergy. The study includes 139 and 250 patients evaluated in Spain and Italy, respectively. All had experienced type I hypersensitivity reactions to penicillins confirmed by positive STs. Additionally, selective or cross-reactive reactions were confirmed by DPTs in a subgroup of patients for further analysis. Positive ImmunoCAP® results were 39.6% for PG and/or AX in Spanish subjects and 52.4% in Italian subjects. When only PG or AX sIgE where analyzed, the percentages were 15.1% and 30.4%, respectively, in Spanish patients; and 38.9% and 46% in Italian ones. The analysis of positive STs showed a statistically significant higher percentage of positive STs to PG determinants in Italian patients. False-positive results to PG (16%) were detected in selective AX patients with confirmed PG tolerance. Low and variable sensitivity values observed in a well-defined population with confirmed allergy diagnosis, as well as false-positive results to PG, suggest that ImmunoCAP® is a diagnostic tool with relevant limitations in the evaluation of subjects with type I hypersensitivity reactions to penicillins Ariza A, Mayorga C, Bogas G, Gaeta F, Salas M, Valluzzi RL, Labella M, Pérez-Sánchez N, Caruso C, Molina A, Fernández TD, Torres MJ, Romano A. Detection of Serum-Specific IgE by Fluoro-Enzyme Immunoassay for Diagnosing Type I Hypersensitivity Reactions to Penicillins. International Journal of Molecular Sciences. 2022; 23(13):6992. https://doi.org/10.3390/ijms23136992 https://hdl.handle.net/10630/24521 https://doi.org/10.3390/ijms23136992 Alergia-Diagnóstico Detection of Serum-Specific IgE by Fluoro-Enzyme Immunoassay for Diagnosing Type I Hypersensitivity Reactions to Penicillins