2026-05-31T06:24:56Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/247602026-02-03T12:12:55Zcom_10630_2254col_10630_37959

00925njm 22002777a 4500 dc Del Espino Pérez, Ángel author López-Márquez, Diego author López-Pagán, Nieves author Rodríguez-Bejarano, Eduardo author Ruiz-Albert, Francisco Javier author Beuzón-López, Carmen del Rosario author 2022 miRNAs are sequence-dependent negative regulators of gene expression involved in many relevant plant processes, including immunity. Activation of defence genes can negatively impact plant fitness, and thus needs to be fine-tuned. miRNA-mediated regulation of gene expression is mediated by the activity of DCL proteins that induce cleavage of target transcripts. We have described miR825-5p as involved in the regulation of immunity. This miRNA specifically targets R genes of the TNL family. We have characterized the regulatory system formed by miRNA825-5p and its main target MIST1. This miRNA only triggers the RDR6-DCL4-dependent production of phasiRNAs from MIST1. MIST1 is the NLR gene from which the most phasiRNAs are produced in Arabidopsis. We reported that pri-miR825 is down-regulated after PAMP-perception and demonstrated that plants with altered levels of miR825-5p exhibit altered PTI-associated phenotypes. In addition, MIST1 has been described to be regulated by other mechanisms like nonsense mediated decay or polyubiquitination. We have characterized the expression pattern of both MIR825 and MIST1 and are currently studying the putative molecular role of MIST1 in defence apart from its demonstrated role as a miRNA825-5p-linked regulatory hub for TNLs regulation in Arabidopsis thaliana. https://hdl.handle.net/10630/24760 Plantas - Defensas - Congresos Ácido ribonucleico - Congresos Arabidopsis thaliana - Congresos Molecular characterization of MicroRNA-silenced TNL-1 (MIST1) and its role in plant defense