2026-05-31T17:00:24Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/247842026-02-03T12:11:18Zcom_10630_2254col_10630_37959

00925njm 22002777a 4500 dc Infantes-López, M. Inmaculada author Zambrana-Infantes, Emma author Chaves-Peña, Patricia author Nieto-Quero, Andrea author Muñoz-Martín, José author Pedraza-Benítez, María del Carmen author Pérez-Martín, Margarita author 2022-07 Stress is the main environmental cause for depression, known to cause brain immune alterations. As major brain immune cells, microglia undergo transcriptional and, consequently, morphological changes that result in tissue damage, including new cell generation impairment. Even so, few brain regions have been thoroughly studied, excluding key regulators as the hypothalamus, in which this process remains partially unknown. Moreover, there is a poor understanding in physiology related to behavioral outcome. Therefore, it would be interesting to study the relationship between microglia and cell proliferation in stressed mice while controlling for behavior. Here, we used the social defeat stress (SDS) paradigm as a depression-inducing protocol in 8-week-old male C57BL/6J mice for 10 consecutive days. Intruder mice behavior was analyzed to assess depression using behavioral tests and K-means clustering. By immunohistochemical and imaging procedures, microglial morphology, and distribution, as well as cell survival, were analyzed in the hypothalamic paraventricular, ventromedial and arcuate nucleus. Finally, statistical mediation analysis was conducted to evaluate the relationship among variables. Results show mice response to SDS was different, being half the mice resilient and half sensitive to depressive-like symptoms. Microglial morphological activation was enhanced in the ventromedial and arcuate nucleus, especially in stress sensitive animals. Similar results were observed in cell survival, which was particularly affected in sensitive mice. Strikingly, these cell survival changes were statistically mediated by microglial activation. As a conclusion, hypothalamic regions were found to respond differently to stress, accordingly to behavioral outcome, in terms of microglial activation and subsequent decrease in cell survival. https://hdl.handle.net/10630/24784 Estrés (Fisiología) - Congresos Depresión mental - Efectos del estrés - Congresos Experimentación animal - Congresos Microglia - Efectos del estrés - Congresos Social defeat stress induces microglial alterations and impaired cell survival in the hypothalamus according to behavioral phenotype