2026-05-28T17:46:35Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/247852026-02-03T12:01:42Zcom_10630_2254col_10630_37959
Effects of chronic stress on hippocampal microglia and neurogenesis of mice under social defeat stress.
Muñoz-Martín, José
Infantes-López, M. Inmaculada
Chaves-Peña, Patricia
Nieto-Quero, Andrea
Zambrana-Infantes, Emma
Pedraza-Benítez, María del Carmen
Pérez-Martín, Margarita
Estrés (Fisiología) - Congresos
Depresión mental - Efectos del estrés - Congresos
Experimentación animal - Congresos
Microglia - Efectos del estrés - Congresos
Introduction: Chronic stress is the main environmental factor in the aetiology of depression and
it is known that this type of stress may cause alterations in brain regions such as the
hippocampus. Nevertheless, changes in a cellular basis are still a subject of study. Objective: The
analysis of microglial cells and immature neurons in the dentate gyrus (DG) of stressed mice.
Methods: C57BL/6J mice were subjected to Social Defeat Stress model (SDS), consisting of 6
days of social isolation prior to 10 days of stressor. The DG was analysed using
immunohistochemistry techniques against Iba1 (microglia) and DCX (immature neurons)
following image analysis (ImageJ) to obtain morphological and distribution data of microglial
somas. Furthermore, hippocampal neurogenesis was assessed through stereological
quantification of DCX+ cells (Visiopharm). Results: An increase in soma size under chronic stress
conditions was shown, as well as a less circular and more ameboid soma. These changes were
observed mainly in the infrapyramidal blade of the DG. According to microglial cells distribution
parameters, the granular cell layer (GCL) was the region which presented the highest microglial
density under SDS. Regarding hippocampal neurogenesis, a decrease in the number of DCX+
Type 2-3 cells was observed in the whole DG. Conclusion: All these results o!er a more profound
insight of stress changes at a cellular level and could contribute to a better understanding of
neurobiological basis in pathologies such as depression. Projects: PSI2017-83408-P (MINECO)
and P20 00460 (Consejería de Conocimiento, Investigación y Universidades, Junta de Andalucía).
2022-07-26T10:40:51Z
2022-07-26T10:40:51Z
2022-07-26T10:40:51Z
2022-07
conference output
https://hdl.handle.net/10630/24785
eng
FENS-FORUM 2022
PARIS, FRANCIA
9-13 JULIO 2022
PID2020-117464RB I00/AEI/10.13039/501100011033
UMA20- FEDERJA-112 (FEDER/Junta de Andalucía)
P20-00460 (Consejería de Conocimiento, Investigación y Universidades, Junta de Andalucía)
PSI2017-83408-P (FEDER/Ministerio de Ciencia, Innovación y Universidades, Spain)
Ministerio de Ciencia e Innovación – Plan Nacional I+D+I from
Spain (PID2020-117464RB I00/AEI/10.13039/501100011033) to CP and MP-M; FEDER/
Ministerio de Ciencia e Innovación – Agencia Estatal de Investigación Investigación from
Spain (PSI2017-83408-P) to CP;
FEDER/Junta de Andalucía – Proyectos I+D+I en el marco del Programa Operativo FEDER
Andalucía 2014-2020 (UMA20-FEDERJA-112) to CP and MP-M; Consejería de Conocimiento,
Investigación y Universidades, Junta de Andalucía (P20_00460) to CP. PID2020-117464RB-I00
Ministerio de Universidades from Spain: FPI (PID2020-117464RB-I00) to CP-P, FPU16/05308 to
AN-Q and FPU19/03629 to MII-L.
open access