2026-06-04T05:40:09Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/249072026-02-03T12:07:20Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Morello-López, Jorge
author
Salas-Liñán, Joaquín J
author
Ruiz-López, Noemí
author
Huércano Rubens, Carolina
2022-09-06T10:35:23Z
2022-09-06T10:35:23Z
2022-08
https://hdl.handle.net/10630/24907
Plants are sessile organisms and therefore they have perfected a complex molecular signalling network to detect and respond to the different environmental stresses such as high temperatures, salinity, or drought. In plants, fatty acid synthesis takes place at chloroplasts, and they are assembled into glycerolipids and sphingolipids at the endoplasmic reticulum (ER). Then, the newly synthetized lipids in the ER are delivered to chloroplast via a non-vesicular pathway, likely through lipid transport proteins (LTP). These LTP would be localized in ER-chloroplast membrane contact sites (MCS).
Synaptotagmin-like mitochondrial-lipid-binding (SMP) domain proteins are evolutionarily conserved LTP in eukaryotes that localize at MCS. They are involved in tethering of these MCS through interaction with other proteins/membrane lipids and in transferring of glycerolipids between these two membranes. We have studied the occurrence of SMP proteins in A. thaliana and S. lycopersicum by searching remote orthologs of human E-Syt1 (SMP protein). By using transient expression in N. benthamiana leaves and confocal microscopy, we have identified the NTMC2T5 family with two homologs in A. thaliana and only one in S. lycopersicum that are anchored to the chloroplast outer membrane and are interacting with the ER (at ER-chloroplast MCS).
Our preliminary data have unequivocally demonstrated that NTMC2T5 proteins are anchored to the chloroplast, and they bind in trans the ER. Additionally, it is predicted that these proteins contain a SMP domain which is a lipid-transfer domain, indicating that these proteins could be responsible for some of the lipid transferring events at ER-chloroplast MCS that are still unknown. Our preliminary phenotypic analyses have shown that these proteins are involved in salt tolerance. Finally, we have observed that clustering of chloroplasts around the nucleus occurred when we overexpressed these proteins in Nicotiana benthamiana leaves.
spa
Cloroplastos
NTMC2T5 protein family: newly identified ER-chloroplast contact site proteins involved in abiotic stress.
conference output
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URL
https://riuma.uma.es/bitstreams/0a5a9a3a-9d3e-49ee-93df-59a176b2441f/download
File
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57fdb86f33fbf5883b0381e3cb5f4f99
78594
application/pdf
24907.pdf