2026-06-02T04:53:41Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/250882026-02-03T11:47:05Zcom_10630_2254col_10630_37959
Novel molecularly imprinted impedimetric biosensor based on polypyrrole and decorated graphene oxide for the routine monitoring of Lysozyme
Montoro-Leal, Pablo
Frías, Isaac A.M.
Vereda-Alonso, Elisa Isabel
Errachid, Abdelhamid
Jaffrezic-Renault, Nicole
Biosensores - Congresos
Lisozima - Congresos
Grafeno - Uso en diagnóstico - Congresos
In this work, a novel molecularly imprinted polymer based on polypyrrole (PPy) and decorated graphene oxide (GO@Fe3O4) was developed for the sensitive detection of lysozyme (LYS). The synthesized material (MIPPy/GO@Fe3O4) was electrodeposited with LYS as a template on gold microelectrodes. Transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS) were used to confirm the adequate preparation of GO@Fe3O4, and the characterization of the resulting microsensors was carried out with the following analytical techniques: electrochemical impedance spectrometry (EIS), FT-IR analysis and scanning electron microscopy (SEM). An equivalent circuit was suggested to quantitatively analyse each component of the sensor system. EIS was also used for the determination of LYS in a wide linear range from 1 to 1 105 pg/mL, presenting good precision (RSD ≈ 10%, n = 5) and low limits of detection and quantification (LOD = 0.009 pg/mL and LOQ = 0.9 pg/mL, respectively). Meanwhile, the microsensor showed a high sensitivity, a good selectivity and reproducibility. The construction process was relatively simple, and provided a rapid and economical method for the routine monitoring of LYS. The microsensor was successfully applied for the detection of this protein in fresh chicken-egg white sample and commercial drug.
2022-09-27T08:04:46Z
2022-09-27T08:04:46Z
2022-09-26
2022-09
conference output
https://hdl.handle.net/10630/25088
eng
EuChemS2022
Lisboa (Portugal)
Del 31 de agosto al 1 de septiembre de 2022
http://creativecommons.org/licenses/by-nc-nd/4.0/
open access
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
EuChem2022