2026-06-02T02:15:32Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/251192026-02-03T11:49:18Zcom_10630_2254col_10630_37959

00925njm 22002777a 4500 dc Molina-Santiago, Carlos author Vela-Corcía, David author Petras, Daniel author Díaz-Martínez, Luis author Pérez-Lorente, Alicia author Pearson, John R. author Caraballo-Rodríguez, Andrés Mauricio author Dorrestein, Pieter author De-Vicente-Moreno, Antonio author Romero-Hinojosa, Diego Francisco author 2022 Bacterial communities are continuously adapting and evolving for survival. They produce and secrete a broad range of molecules that kill, defend, or mediate communication between cells of different lineages, thus shaping the final structure of the microbial community. In this work, with the combination of -omics approaches, molecular biology and microscopic techniques, we expand our knowledge on the chemical interplay and specific mutations that modulate the transition from antagonism to co-existence between two plant-beneficial bacteria, Pseudomonas chlororaphis and Bacillus amyloliquefaciens. We demonstrate that bacillaene, a secondary metabolite with bacteriostatic activity produced by Bacillus, interacts with the protein elongation factor FusA of P. chlororaphis to arrest its growth and population advancement. Point mutations in this protein lead to tolerance to bacillaene and other inhibitors of protein translation. Additionally, we describe the key role of the glycerol kinase GlpK from B. amyloliquefaciens in its unspecific tolerance against P. chlororaphis. Mutations in GlpK provoked by a decrease of Bacillus cell membrane permeability among other pleiotropic responses. We conclude that nutrient specialization and mutations in basic biological functions are bacterial adaptive dynamics that lead to the coexistence of two primary competitive bacterial species rather than their mutual eradication. https://hdl.handle.net/10630/25119 Bacillus (Bacteria) Pseudomonas From bacterial antagonism to co-existence: the chemical interplay and adaptative strategies between Pseudomonas and Bacillus