2026-06-05T12:15:58Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/252612026-02-03T12:32:03Zcom_10630_2254col_10630_37959

The potential of the RNAi technology, via SIGS, in the control of Botrytis cinerea in horticultural crops López-Laguna, Alba Vielba-Fernández, Alejandra Pérez-García, Alejandro Fernández-Ortuño, Dolores Microbiología agrícola Hongos en agricultura Botrytis cinerea RNAi fungicidas dsRNA Hortícolas Botrytis cinerea is one of the most important limiting factors for crop production worldwide, as it demonstrated by the enormous annual intake of fungicides used for their control to avoid crop losses. However, this fungus has been categorized by FRAC as a high-risk pathogen for fungicide resistance development. Another problem is related with the diversity of fungicides available to growers, which according with the current European legislation on pesticides and the European Green Deal, will be reduced by 50% by 2030. For all this, new low-impact sustainable solutions, obtained through new phytoprotection tools, to control B. cinerea are needed. In this study, we intend to check if some emerging strategies such as RNA interference technology (RNAi) could be valid sustainable solution and alternative to the use of conventional chemical fungicides for the control of B. cinerea in crops of relevance. To achieve this goal, the SIGS (spray-induced gene silencing) approach, which concerns the exogenous application of double-stranded RNA (dsRNA), was tested. For it, ten double-stranded RNA (dsRNAs) were designed against the fungicide target’s genes and genes encoding proteins involved in virulence/pathogenicity of this fungus. The preliminary results obtained in in vitro tests have shown that the application of the different dsRNAs, individually and in combination, have significantly reduced the development of the fungus on different culture media. In addition, this reduction was very promising on detached fruit and in planta assays, demonstrating the potential of this technique in the control of B. cinerea. On the other hand, the sustained release of the dsRNA-fungicides using nanoparticles as a carrier or stabilizer has also been analyzed. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. 2022-10-20T07:31:58Z 2022-10-20T07:31:58Z 2022-06 conference output https://hdl.handle.net/10630/25261 eng Botrytis-Sclerotinia Symposium 2022 Avignon, Francia 13-17 junio 2022 open access application/pdf