2026-06-01T16:38:50Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/263412026-02-03T10:52:55Zcom_10630_2254col_10630_37953
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Valderrama-Martín, José Miguel
author
Ortigosa Peña, Francisco
author
Aledo-Ramos, Juan Carlos
author
Ávila-Sáez, Concepción
author
Cánovas-Ramos, Francisco Miguel
author
Cañas-Pendón, Rafael Antonio
2023-04-21T09:44:19Z
2023-04-21T09:44:19Z
2023
Valderrama‐Martín, Ortigosa, F., Aledo, J. C., Ávila, C., Cánovas, F. M., & Cañas, R. A. (2023). Pine has two glutamine synthetase paralogs, GS1b.1 and GS1b.2, exhibiting distinct biochemical properties. The Plant Journal : for Cell and Molecular Biology, 113(6), 1330–1347. https://doi.org/10.1111/tpj.16113
https://hdl.handle.net/10630/26341
https://doi.org/10.1111/tpj.16113
The enzyme glutamine synthetase (EC 6.3.1.2) is mainly responsible for the incorporation of inorganic nitrogen into organic molecules in plants. In the present work, a pine (Pinus pinaster) GS1 (PpGS1b.2) gene was
identified, showing a high sequence identity with the GS1b.1 gene previously characterized in conifers. Phylogenetic analysis revealed that the presence of PpGS1b.2 is restricted to the genera Pinus and Picea and is
not found in other conifers. Gene expression data suggest a putative role of PpGS1b.2 in plant development, similar to other GS1b genes from angiosperms, suggesting evolutionary convergence. The characterization of GS1b.1 and GS1b.2 at the structural, physicochemical, and kinetic levels has shown differences
even though they have high sequence homology. GS1b.2 had a lower optimum pH (6 vs. 6.5) and was less
thermally stable than GS1b.1. GS1b.2 exhibited positive cooperativity for glutamate and substrate inhibition
for ammonium. However, GS1b.1 exhibited substrate inhibition behavior for glutamate and ATP. Alterations
in the kinetic characteristics produced by site-directed mutagenesis carried out in this work strongly suggest an implication of amino acids at positions 264 and 267 in the active center of pine GS1b.1 and GS1b.2
being involved in affinity toward ammonium. Therefore, the amino acid differences between GS1b.1 and
GS1b.2 would support the functioning of both enzymes to meet distinct plant needs
eng
Atribución-NoComercial 4.0 Internacional
Bioquímica
Glutamina
Metabolismo
Pine has two glutamine synthetase paralogs, GS1b.1 and GS1b.2, exhibiting distinct biochemical properties
journal article
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The Plant Journal - 2023 - Valderrama‐Mart n - Pine has two glutamine synthetase paralogs GS1b 1 and GS1b 2 exhibiting.pdf