2026-06-02T15:21:23Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/269582026-02-03T11:31:07Zcom_10630_2254col_10630_37953
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Caballano-Infantes, Estefanía
author
Ho-Plágaro, Ailec
author
López-Gómez, Carlos
author
Martín-Reyes, Flores
author
Rodríguez-Pacheco, Francisca
author
Taminiau, Bernard
author
Daube, Georges
author
Garrido Sánchez, Lourdes
author
Alcaín-Martínez, Guillermo
author
Andrade-Bellido, Raúl Jesús
author
García-Cortés, Miren
author
Lucena-González, María Isabel
author
García Fuentes, Eduardo
author
Rodriguez-Diaz, Cristina
2023-06-15T07:36:36Z
2023-06-15T07:36:36Z
2023-03-27
Caballano-Infantes E, Ho-Plágaro A, López-Gómez C, Martín-Reyes F, Rodríguez-Pacheco F, Taminiau B, Daube G, Garrido-Sánchez L, Alcaín-Martínez G, Andrade RJ, et al. Membrane Vesicles of Toxigenic Clostridioides difficile Affect the Metabolism of Liver HepG2 Cells. Antioxidants. 2023; 12(4):818. https://doi.org/10.3390/antiox12040818
https://hdl.handle.net/10630/26958
https://doi.org/10.3390/antiox12040818
Clostridioides difficile infection (CDI) appears to be associated with different liver diseases. C. difficile secretes membrane vesicles (MVs), which may be involved in the development of nonalcoholic fatty liver disease (NALFD) and drug-induced liver injury (DILI). In this study, we investigated the presence of C. difficile-derived MVs in patients with and without CDI, and analyzed their effects on pathways related to NAFLD and DILI in HepG2 cells. Fecal extracellular vesicles from CDI patients showed an increase of Clostridioides MVs. C. difficile-derived MVs that were internalized by HepG2 cells. Toxigenic C. difficile-derived MVs decreased mitochondrial membrane potential and increased intracellular ROS compared to non-toxigenic C. difficile-derived MVs. In addition, toxigenic C. difficile-derived MVs upregulated the expression of genes related to mitochondrial fission (FIS1 and DRP1), antioxidant status (GPX1), apoptosis (CASP3), glycolysis (HK2, PDK1, LDHA and PKM2) and β-oxidation (CPT1A), as well as anti- and pro-inflammatory genes (IL-6 and IL-10). However, non-toxigenic C. difficile-derived MVs did not produce changes in the expression of these genes, except for CPT1A, which was also increased. In conclusion, the metabolic and mitochondrial changes produced by MVs obtained from toxigenic C. difficile present in CDI feces are common pathophysiological features observed in the NAFLD spectrum and DILI.
eng
Atribución 4.0 Internacional
Células hepáticas
Metabolismo
Vesícula biliar
Aparato digestivo-Enfermedades
Patógenos
Patología celular
Membrane vesicles of toxigenic Clostridioides difficile affect the metabolism of liver hepG2 cells
journal article
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URL
https://riuma.uma.es/bitstreams/8bf43dbe-027b-4ec7-92bc-74a4f8167886/download
File
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f30db40901975e67d48742bc386248e1
5286130
application/pdf
antioxidants-12-00818.pdf