2026-06-01T12:32:35Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/271732026-02-03T11:48:22Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Bakhat, Nisrine
author
Fernández-Ortuño, Dolores
author
Pérez-García, Alejandro
2023-07-05T08:39:05Z
2023-07-05T08:39:05Z
2023
https://hdl.handle.net/10630/27173
Fungal pathogens are the main destructive microorganisms for terrestrial plants and pose increasing challenges for global agricultural production. Chitin is a vital building block for fungal cell walls and a widely effective inducer for plant immunity. Chitin-triggered immunity is a powerful plant defense response against fungi. Therefore, phytopathogenic fungi have developed different virulence factors that allow them to suppress the activation of this defensive response. In this study, we intend to evaluate the molecular mechanisms of suppression the chitin-triggered previously identified in Podosphaera xanthii, the main causal agent of powdery mildew in cucurbits. These mechanisms consist of the modification of chitin immunogenic oligomers (CDA), the binding to these oligomers (CHBE) and their degradation (EWCAs). For this, we used RNA interference (RNAi) technology, which consists of the application of double-stranded RNA (dsRNA) designed to suppress the expression of the PxCDA and PxEWCA genes, which would result in the reduction of the three mechanisms of suppression of chitin signaling mentioned above, since CDA and CHBE proteins are products of the same PxCDA gene. Application of dsRNA was carried out using leaf disc assays and infiltration of melon cotyledons. The preliminary results obtained indicate that the application of dsRNA significantly reduces the development of the fungus and the symptoms of powdery mildew disease in melon, suggesting that chitin signaling suppression mechanisms are essential for the development of P. xanthii.
eng
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Quitina
Hongos fitopatógenos
Inmunología vegetal
The combined function of Podosphaera xanthii's mechanisms to suppress immunity triggered by chitin.
conference output
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URL
https://riuma.uma.es/bitstreams/28091aae-7368-4580-ba87-ba6e2cd9f2ab/download
File
MD5
f5fddae80c7a7ebbe2a3095476f259af
557897
application/pdf
Abstract SEM.pdf