2026-06-06T02:40:22Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/272162026-02-03T11:11:06Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Rubio-Valverde, Lourdes author Díaz-García, Jordi author Martín-Pizarro, Carmen author Siverio, José M. author Raven, John A. author Fernández-García, José Antonio author 2023-07-05 Zostera marina is a seagrass, a group of angiosperms that evolved from land to live submerged in seawater, an environment of high salinity, alkaline pH and usually very low NO3−. In 2000, we reported the first physiological evidence for the Na+-dependent high-affinity NO3− uptake in this plant. Now, to determine the molecular identity of this process, we searched for NO3− transporters common to other vascular plants encoded in Z. marina's genome. We cloned two candidates, ZosmaNPF6.3 and ZosmaNRT2 with its partner protein ZosmaNAR2. ZosmaNAR2 expression levels increase up to 4.5-fold in Z. marina leaves under NO3−-deficiency, while ZosmaNRT2 and ZosmaNPF6.3 expressions were low and unaffected by NO3−. NO3− transport capacity, kinetic properties and H+ or Na+-dependence were examined by heterologous expression in the Hansenula polymorpha high-affinity NO3− transporter gene disrupted strain (∆ynt1). ZosmaNPF6.3 functions as a H+-dependent NO3− transporter, without functionality at alkaline pH and apparent dual kinetics (KM = 11.1 µM at NO3− concentrations below 50 µM). ZosmaNRT2 transports NO3− in a H+-independent but Na+-dependent manner (KM = 1 mM Na+), with low NO3− affinity (KM = 30 µM). When ZosmaNRT2 and ZosmaNAR2 are co-expressed, a Na+-dependent high-affinity NO3− transport occurs (KM = 5.7 µM NO3−), mimicking the in vivo value. These results are discussed in the physiological context, providing evidence that ZosmaNRT2 is a Na+-dependent high-affinity NO3− transporter, the first of its kind to be functionally characterised in a vascular plant, that requires ZosmaNAR2 to achieve the necessary high-affinity for nitrate uptake from seawater. Rubio L, Díaz-García J, Martín-Pizarro C, Siverio JM, Raven JA, Fernández JA. Identity and functional characterisation of the transporter supporting the Na+ -dependent high-affinity NO3- uptake in Zostera marina L. Plant Cell Environ. 2023 Jul 5. doi: 10.1111/pce.14660. https://hdl.handle.net/10630/27216 10.1111/pce.14660 Zostera marina Flora marina Angiospermas Nitratos Biología molecular vegetal Identity and functional characterisation of the transporter supporting the Na+-dependent high-affinity NO3− uptake in Zostera marina L.