2026-05-31T02:39:35Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/273912026-02-03T11:54:33Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
González-García, Marta
author
Carrillo-Franco, Laura
author
Morales-Luque, Carmen
author
Díaz-Casares, Amelia
author
Ponce-Velasco, Marina
author
Gago-Calderón, Belén
author
Dawid-Milner, Marc Stefan
author
López-González, Manuel Víctor
2023-07-28T11:21:27Z
2023-07-28T11:21:27Z
2023-07
https://hdl.handle.net/10630/27391
The stimulation of the Periaqueductal Gray matter (PAG) and nucleus retroambiguus (nRA) produces vocalization. A high expression of FOXP2 protein at mesencephalic (PAG) and pontine regions involved in cardiorespiratory control has been described. The aim of this study was to characterize the possible role of the dlPAG in modulating laryngeal activity and their effects on vocalization.
Experimental studies were carried out with non-inbred male rats (n=27), Sprague-Dawley (250-300 g). Animals were anesthetized with sodium pentobarbitone (60 mg/kg i.p., initial dose, supplemented 2mg/ kg, i.v., as necessary).
Neuromorphological study (n=6)
The pattern of staining for c-Fos and FOXP2 protein immunoreactivity (c-Fos-ir) were examinated throughout the rostrocaudal extent of the nRa/nA region during electrical stimulation of the dlPAG.
Neuropharmacological study (n=21)
A double tracheal cannulation was used to obtain an “isolated glottis in situ” and to record respiratory airflow. Bilateral parietostomy allowed access to the dlPAG. Electrical stimulations (n=7) of this region using concentric bipolar electrodes (1ms pulses, 20-40µA, 100Hz for 5s) were performed. Microinjections of PBS-Evans Blue (250nl, pH 7.4±0.1, 5-s duration) (n=7) or glutamate (0,25M, 250nl) (n=7) were performed. Respiratory flow, pleural pressure, blood pressure and heart rate were also recorded.
Activation of the dlPAG elicited a selective increase in c-Fos-ir with an ipsilateral predominance in nRA/nA somatas (p<0.01) and confirm the expression of FOXP2 bilaterally in both nuclei. dlPAG PBS-Evans Blue microinjections did not produce any significant changes in any of the cardiorespiratory variables recorded. dlPAG stimulations evoked a decrease of laryngeal resistance (subglottal pressure) (p<0,001) accompanied with an inspiratory facilitatory response consisted of an increase in respiratory rate (p<0,001), together with a pressor (p<0,001) and tachycardic response (p<0,001).
eng
Fisiología comparada
Laryngeal effects of stimulation of the dorsolateral Periaqueductal Grey Matter in spontaneously breathing anaesthetized rats
conference output
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URL
https://riuma.uma.es/bitstreams/5348b8b2-ca1e-4aff-a891-f9ece3c1e97c/download
File
MD5
60f7494aac8f87057ad633afc61184a9
195324
application/pdf
dlPAG and LARYNX PHYSOC 2023.pdf