2026-06-06T05:02:05Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/280492026-02-03T12:18:05Zcom_10630_2254col_10630_37959

00925njm 22002777a 4500 dc Mancera-Miranda, Laura author López-Pagán, Nieves author Rufián Plaza, José Sebastián author Sánchez-Romero, María Antonia author Gutierrez-Pozo, Gabriel author Spröer, Cathrin author Bunk, Boyke author Ruiz-Albert, Francisco Javier author Beuzón-López, Carmen del Rosario author 2023 Phenotypic heterogeneity usually refers to the co-existance of different phenotypes within a population. Phenotypic differences may arise through genetic variation (genomic rearrangements or mutations) or through the response to differences in the stimuli as encountered within the microenvironment. But also, sometimes, the sources of variation may not be deterministic, i.e. directly related to stimuli, but a consequence of molecular noise in gene expression and/or a programmed event under genetic or epigenetic control. A particular example of phenotypic heterogeneity is bistability. Bistability occurs when a bacterial clonal population splits into two subpopulations showing distinct phenotypes. Phenotypic heterogeneity can be beneficial in fluctuating environments by allowing some individuals within the clonal population to survive sudden changes (risk-spreading). It can also benefit the entire population through cooperation between individuals displaying phenotypic differences (division of labour). These processes and their biological relevance have been described in some animal pathogens, but little is known about them in plant-pathosystems. Pseudomonas syringae is a plant-pathogenic bacterium whose virulence depends on the expression of a type III secretion system (T3SS). Our team has previously reported that T3SS expression is bistable under inducing conditions, generating two subpopulations (T3SSON/T3SSOFF) that show differences in virulence. We have identified other loci including genes related to motility, biofilm formation and DNA methylation that also display phenotypic heterogeneity to very different degrees and with different dynamics and are at different stages on their molecular and biological characterization. Our latest advances on this front will be presented and discussed. https://hdl.handle.net/10630/28049 Bacterias fitopatógenas Pseudomonas syringae - Genética Phenotypically heterogeneous loci in the plant pathogen Pseudomonas syringae.