2026-05-28T09:32:00Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/282962026-02-03T12:22:17Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Escalante Quirós, Sandra
author
Rojas Pérez, Jennifer
author
Csukasi, Fabiana
author
Durán-Jimenez, Iván Jesús
2023-12-14T09:58:46Z
2023-12-14T09:58:46Z
2023
https://hdl.handle.net/10630/28296
Decellularized bone matrix ( is a classic approach in bone tissue engineering based on the removal of bone tissue cells and the
calcified phase using chemical, physical o enzymatic agents The resulting matrix preserves its three dimensional structure and biomechanical properties, providing a native microenvironment suitable for osteogenic development However, due to the complexity of the bone structure, with this method has not been possible to obtain an experimentally reproducible bone organoid that sufficiently replicates the bone biology Another approach to generate organoids is the use of hydrogels functionalized with adhesion peptides and morphogenetic proteins that favor the differentiation and osteogenic capacity of the organoid In this work we developed a new bone model based on the combination of hydrogels and DBMs We tested PVA and dextran hydrogels in combination with BMP responsive reporter cell line ( and the long term effect of the RGD peptide addition to hydrogels evaluating osteogenic differentiation by alkaline phosphatase method and luciferase assays We analyze by histology decellularized DBMs combined with dextran hydrogels containing BRITER cells cultured under osteogenic conditions for 3 weeks Our results showed that this double scaffolding is able to support osteogenic differentiation as well as osseointegration, proving its potential for bone organoid generation
eng
Organos (Anatomía) - Cultivo
Huesos
Coloides
Bone organoid generation based on double scaffolding.
conference output
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URL
https://riuma.uma.es/bitstreams/c333be67-ef10-47b7-ac10-5eac176556c4/download
File
MD5
1c6664aa17aefc50726767060f848bc2
1087806
application/pdf
Poster_Base SEBC 2023 2.pdf