2026-06-03T00:52:11Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/289132026-02-03T11:25:17Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Negrini, Joao author Mozos, Elena author Escamilla-Sánchez, Alejandro author Pérez, José author Lucena, Rosario author Guerra, Rafael author Ginel, Pedro author 2017-06-06 Compared with mammals, wound healing in reptiles is characterized by reduced wound contraction and longer healing times. The aim of this study is to describe the clinical and histopathological effects of topical insulin on second-intention healing of experimentally induced wounds in skin without dermal bony plates of Trachemys scripta elegans exposed to daily variations in ambient temperature and in an aquatic environment. Forty-four healthy adult females were assigned to two groups: Group 1 (n = 24) was used to assess clinical features such as wound contraction; Group 2 (n = 20) was used for histological evaluation and morphometric analysis. Topical porcine insulin (5 IU/ml diluted in glycerol) was applied daily 1 week. For each control time (2, 7, 14, 21 and 28 days post-wounding), re-epithelisation and wound remodelling were evaluated histologically and the number of main inflammatory cells (heterophils, macrophages, lymphocytes and fibroblasts) was scored. Results Mean wound contraction was higher in the insulin-treated group at each time point and differences were significant at day 28 (P < 0.0001). Histologically, these clinical findings were associated with better re-epithelisation, inflammatory response, collagen synthesis and remodelling of the wounds. Morphometrically, insulin-treated wounds had significantly higher mean counts of heterophils (day 7), macrophages (days 2, 7 and 14) and fibroblasts (days 14 and 21), whereas lymphocyte counts were significantly lower at day 21. These results demonstrate that topical insulin modifies the inflammatory response of turtle skin up-regulating inflammatory cells at early stages and promoting wound healing. Conclusions Topical insulin is a potentially useful therapy in skin wounds of Trachemys scripta and should be evaluated in non-experimental wounds of turtles and other reptiles. Negrini, J., Mozos, E., Escamilla, A. et al. Effects of topical insulin on second-intention wound healing in the red-eared slider turtle (Trachemys scripta elegans) – a controlled study. BMC Vet Res 13, 160 (2017). https://doi.org/10.1186/s12917-017-1082-8 https://hdl.handle.net/10630/28913 10.1186/s12917-017-1082-8 Cicatrización Effects of topical insulin on second-intention wound healing in the red-eared slider turtle (Trachemys scripta elegans) – a controlled study