2026-05-31T02:36:25Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/292642026-02-03T10:59:27Zcom_10630_2254col_10630_37953
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Claros-Díaz, Manuel Gonzalo
author
Herrera‐Uribe, Juber
author
Zaldívar‐López, Sara
author
Anaya-Aguilar, Carmen
author
Luque, Cristina
author
Bautista-Moreno, Rocío
author
Carvajal, Ana
author
Garrido, Juan José
2024-01-26T09:49:57Z
2024-01-26T09:49:57Z
2018
Herrera-Uribe J, Zaldívar-López S, Aguilar C, Luque C, Bautista R, Carvajal A, Claros MG, Garrido JJ. Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection. Vet Res. 2018 Feb 1;49(1):9. doi: 10.1186/s13567-018-0506-1
https://hdl.handle.net/10630/29264
10.1186/s13567‐018‐0506‐1
Salmonellosis is a gastrointestinal disease caused by non‐typhoidal Salmonella serovars such as Salmonella Typhimu‐ rium. This pathology is a zoonosis, and food animals with subclinical infection constitute a vast reservoir for disease. After intestinal colonization, Salmonella Typhimurium reaches mesenteric lymph nodes (MLN), where infection is controlled avoiding systemic spread. Although the molecular basis of this infection has been extensively studied, little is known about how microRNA (miRNA) regulate the expression of proteins involved in the Salmonella‐host interac‐ tion. Using small RNA‐seq, we examined expression profiles of MLN 2 days after infection with Salmonella Typhimu‐ rium, and we found 110 dysregulated miRNA. Among them, we found upregulated miR‐21, miR‐155, miR‐150, and miR‐221, as well as downregulated miR‐143 and miR‐125, all of them previously linked to other bacterial infections. Integration with proteomic data revealed 30 miRNA potentially regulating the expression of 15 proteins involved in biological functions such as cell death and survival, inflammatory response and antigenic presentation. The inflamma‐ tory response was found increased via upregulation of miRNA such as miR‐21 and miR‐155. Downregulation of miR‐ 125a/b, miR‐148 and miR‐1 were identified as potential regulators of MHC‐class I components PSMB8, HSP90B1 and PDIA3, respectively. Furthermore, we confirmed that miR‐125a is a direct target of immunoproteasome component PSMB8. Since we also found miR‐130 downregulation, which is associated with upregulation of HSPA8, we suggest induction of both MHC‐I and MHC‐II antigen presentation pathways. In conclusion, our study identifies miRNA that could regulate critical networks for antigenic presentation, inflammatory response and cytoskeletal rearrangements.
eng
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Salmonelosis
Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection
journal article
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1d0b0a042291d8bb05e894da6365a34a
2328926
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VetRes2018.pdf