2026-06-02T10:39:18Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/294632026-02-03T11:30:48Zcom_10630_2254col_10630_37953

A5 region modulation of the cardiorespiratory responses evoked from parabrachial cell bodies in the anaesthetised rat Dawid-Milner, Marc Stefan Lara-Muñoz, José Pablo López de Miguel, M Pilar López-González, Manuel Víctor Spyer, KM González-Barón, Salvador Respiración - Regulación - Modelos animales Corazón - Regulación - Modelos animales Experimentación animal We have examined the importance of the A5 region modulating cardiorespiratory responses evoked from the parabrachial complex (PB) in spontaneously breathing rats. Cardiorespiratory changes were analyzed in response to electrical stimulation and glutamate microinjections into the PB (10–20 nl, 1–2 nmol) before and after ipsilateral microinjection of muscimol (50 nl, 0.25 nmol) or lidocaine (50 nl, 0.5 nmol) within the A5 region. Stimulation of medial parabrachial and Ko¨lliker–Fuse nuclei (mPB-KF) evoked a decrease in respiratory rate (P,0.001) with a rise in blood pressure (P,0.001) and heart rate (P,0.05). After muscimol or lidocaine microinjections within the A5 region, the pressor and heart rate responses to mPB-KF stimulation were reduced (P,0.05, both cases). Muscimol within the A5 region altered the respiratory response to glutamate stimulation of mPB-KF, evoking an increase in respiratory rate (P,0.05). Lidocaine abolished the respiratory response to mPB-KF stimulation. Stimulation of the lateral parabrachial nuclei (lPB) caused an increase in respiratory rate (P,0.001) with a rise in blood pressure (P,0.001) and heart rate (P,0.05). Muscimol or lidocaine microinjections within A5 region decreased heart rate (P,0.05) and pressor responses (P,0.05) evoked from lPB. The increase of respiratory rate persisted unchanged. To confirm functional interactions between A5 and PB, extracellular recordings of putative A5 neurones were obtained during PB stimulation. Eighty-three A5 cells were recorded, 35 were activated from the mPB-KF (42%). The results indicate that neurones of the A5 region participate in the cardiorespiratory response evoked from the different regions of the PB complex. The possible mechanisms involved in these interactions are discussed 2024-01-31T09:35:19Z 2024-01-31T09:35:19Z 2003 journal article Dawid Milner MS, Lara JP, López de Miguel MP, López-González MV, Spyer KM, González-Barón S. A5 region modulation of the cardiorespiratory responses evoked from parabrachial cell bodies in the anaesthetised rat. Brain Res. 2003 Aug 22;982(1):108-18. doi: 10.1016/s0006-8993(03)03005-1 https://hdl.handle.net/10630/29463 doi: 10.1016/s0006-8993(03)03005-1 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier