2026-06-02T03:51:09Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/295262026-02-03T10:52:40Zcom_10630_2254col_10630_37953
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
López-González, Manuel Víctor
author
González-García, Marta
author
Peinado-Aragonés, Carlos Antonio
author
Barbancho-Fernández, Miguel Ángel
author
Díaz-Casares, Amelia
author
Dawid-Milner, Marc Stefan
2024-01-31T12:50:10Z
2024-01-31T12:50:10Z
2020
López-González, M.V., González-García, M., Peinado-Aragonés, C.A. et al. Pontine A5 region modulation of the cardiorespiratory response evoked from the midbrain dorsolateral periaqueductal grey. J Physiol Biochem 76, 561–572 (2020). https://doi.org/10.1007/s13105-020-00761-1
https://hdl.handle.net/10630/29526
doi: 10.1007/s13105-020- 00761-1
Connections between the midbrain dorsolateral periaqueductal grey (dlPAG) and the pontine A5 region have been shown. The stimulation of both regions evokes similar cardiovascular responses: tachycardia and hypertension. Accordingly, we have studied the interactions between dlPAG and A5 region in spontaneously breathing anesthetized rats. dlPAG was electrically stimulated (20–30 μA 1-ms pulses were given for 5 s at 100 Hz). Changes in the evoked cardiorespiratoy response were analysed before and after ipsilateral microinjections of muscimol (GABAergic agonist, 50 nl, 0.25 nmol, 5 s) within the A5 region. Electrical stimulation of the dlPAG produces, in the rat, a response characterized by tachypnoea (p < 0.001), hypertension (p < 0.001) and tachycardia (p < 0.001). The increase in respiratory rate was due to a decrease in expiratory time (p < 0.01). Pharmacological inhibition of the A5 region with muscimol produced a marked reduction of the tachycardia (p < 0.001) and the tachypnoea (p < 0.01) evoked from the dlPAG. Finally, to assess functional interactions between A5 and dlPAG, extracellular activity of putative A5 neurones were recorded during dlPAG electrical stimulation. Forty A5 cells were recorded, 16 of which were affected by dlPAG stimulation (40%). 4 cells showed activation, 5 cells excitation and 7 cells decreased spontaneous activity to dlPAG stimulation (p < 0.001). These results confirm a link between the A5 region and dlPAG. The potential role of these connections in the modulation of dlPAG evoked cardiorespiratory responses and their possible clinical implications are discussed.
eng
Respiración - Regulación - Modelos animales
Corazón - Regulación - Modelos animales
Experimentación animal
Pontine A5 region modulation of the cardiorespiratory response evoked from the midbrain dorsolateral periaqueductal grey
journal article
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