2026-06-05T13:37:43Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/296132026-02-03T10:59:37Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Orozco-Navarrete, Begoña author Kaczmarska, Zuzanna author Dupeux, Florine author Garrido-Arandia, María author Pott, Delphine author Díaz-Perales, Araceli author Casañal, Ana author Márquez, José A. author Valpuesta-Fernández, Victoriano author Merchante-Berg, Catharina author 2019-11-27 Although strawberries are highly appreciated fruits, their intake can induce allergic reactions in atopic patients. These reactions can be due to the patient’s previous sensitization to the major birch pollen allergen Bet v 1, by which IgE generated in response to Bet v 1 cross-reacts with the structurally related strawberry Fra a 1 protein family. Fra a 1.02 is the most expressed paralog in ripe strawberries and is highly allergenic. To better understand the molecular mechanisms regulating this allergic response, we have determined the three-dimensional structure of Fra a 1.02 and four site-directed mutants that were designed based on their positions in potential epitopes. Fra a 1.02 and mutants conform to the START fold. We show that the cross-reactivity of all the mutant variants to IgE from patients allergic to Bet v 1 was significantly reduced without altering the conserved structural fold, so that they could potentially be used as hypoallergenic Fra a 1 variants for the generation of vaccines against strawberry allergy in atopic patients. Orozco-Navarrete B, Kaczmarska Z, Dupeux F, Garrido-Arandia M, Pott D, Perales AD, Casañal A, Márquez JA, Valpuesta V, Merchante C. Structural Bases for the Allergenicity of Fra a 1.02 in Strawberry Fruits. J Agric Food Chem. 2020 Sep 30;68(39):10951-10961. doi: 10.1021/acs.jafc.9b05714. Epub 2020 Jan 3. PMID: 31774998; PMCID: PMC7644122. https://hdl.handle.net/10630/29613 10.1021/acs.jafc.9b05714 Fresas Alergenos Structural Bases for the Allergenicity of Fra a 1.02 in Strawberry Fruits.