2026-05-30T03:14:25Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/296542026-02-03T11:30:11Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Rodríguez-Losada, Noela author Wendelbo, Rune author Ocaña, C author Díaz-Casares, Amelia author Guzmán de Villoria, Roberto author Aguirre-Gómez, José Ángel author Arráez-Sánchez, Miguel Ángel author González-Alegre, P author Medina, MA author Arenas, Ernest author Narváez-Bueno, José Ángel author 2020 Emerging scaffold structures made of carbon nanomaterials, such as graphene oxide (GO) have shown efficient bioconjugation with common biomolecules. Previous studies described that GO promotes the differentiation of neural stem cells and may be useful for neural regeneration. In this study, we examined the capacity of GO, full reduced (FRGO), and partially reduced (PRGO) powder and film to support survival, proliferation, differentiation, maturation, and bioenergetic function of a dopaminergic (DA) cell line derived from the mouse substantia nigra (SN4741). Our results show that the morphology of the film and the species of graphene (GO, PRGO, or FRGO) influences the behavior and function of these neurons. In general, we found better biocompatibility of the film species than that of the powder. Analysis of cell viability and cytotoxicity showed good cell survival, a lack of cell death in all GO forms and its derivatives, a decreased proliferation, and increased differentiation over time. Neuronal maturation of SN4741 in all GO forms, and its derivatives were assessed by increased protein levels of tyrosine hydroxylase (TH), dopamine transporter (DAT), the glutamate inward rectifying potassium channel 2 (GIRK2), and of synaptic proteins, such as synaptobrevin and synaptophysin. Rodriguez-Losada N, Wendelbob R, Ocaña MC, Casares AD, Guzman de Villoría R, Aguirre Gomez JA, Arraez MA, Gonzalez-Alegre P, Medina MA, Arenas E and Narvaez JA (2020) Graphene Oxide and Reduced Derivatives, as Powder or Film Scaffolds, Differentially Promote Dopaminergic Neuron Differentiation and Survival. Front. Neurosci. 14:570409. doi: 10.3389/fnins.2020.570409 https://hdl.handle.net/10630/29654 http://doi.org/10.3389/fnins.2020.570409 Grafeno Graphene oxide and reduced derivates, as powder or film scaffolds, differentially promote dopaminergic neuron differentiation and survival.