2026-05-28T19:58:00Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/301882026-02-03T11:22:33Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Tian, Hui author MacKenzie, Craig author Rodríguez-Moreno, Luis Gabriel author Van den Berg, Grardy author Chen, Hongxin author Rudd, Jason author Mesters, Jeroen author Thomma, Bart author 2021-04-01 Chitin is a major structural component of fungal cell walls and acts as a microbe- associated molecular pattern (MAMP) that, on recognition by a plant host, triggers the activation of immune responses. To avoid the activation of these responses, the Septoria tritici blotch (STB) pathogen of wheat, Zymoseptoria tritici, secretes LysM ef- fector proteins. Previously, the LysM effectors Mg1LysM and Mg3LysM were shown to protect fungal hyphae against host chitinases. Furthermore, Mg3LysM, but not Mg1LysM, was shown to suppress chitin-induced reactive oxygen species (ROS) pro- duction. Whereas initially a third LysM effector gene was disregarded as a presumed pseudogene, we now provide functional data to show that this gene also encodes a LysM effector, named Mgx1LysM, that is functional during wheat colonization. While Mg3LysM confers a major contribution to Z. tritici virulence, Mgx1LysM and Mg1LysM contribute to Z. tritici virulence with smaller effects. All three LysM effectors display partial functional redundancy. We furthermore demonstrate that Mgx1LysM binds chitin, suppresses the chitin-induced ROS burst, and is able to protect fungal hyphae against chitinase hydrolysis. Finally, we demonstrate that Mgx1LysM is able to un- dergo chitin-induced polymerization. Collectively, our data show that Z. tritici utilizes three LysM effectors to disarm chitin-triggered wheat immunity. 14646722 https://hdl.handle.net/10630/30188 10.1111/mpp.13055 Quitina - Efectos fisiológicos Inmunología vegetal Hongos fitopatógenos Three LysM effectors of Zymoseptoria tritici collectively disarm chitin-triggered plant immunity.