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oai:riuma.uma.es:10630/309842026-02-03T11:33:58Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Arrebola-Díez, Eva María author Cazorla-López, Francisco Manuel author Romero-Hinojosa, Diego Francisco author Pérez-García, Alejandro author De-Vicente-Moreno, Antonio author 2007-04-20 Pseudomonas syringae pv. syringae, which causes the bacterial apical necrosis of mango, produces the antimetabolite mangotoxin. We report here the cloning, sequencing, and identity analysis of a chromosomal region of 11.1 kb from strain P. syringae pv. syringae UMAF0158, which is involved in mangotoxin biosynthesis. This chromosomal region contains six complete open reading frames (ORFs), including a large gene (ORF5) with a modular architecture characteristic of nonribosomal peptide synthetases (NRPS) named mgoA. A Tn5 mutant disrupted in mgoA was defective in mangotoxin production, revealing the involvement of the putative NRPS gene in the biosynthesis of mangotoxin. This derivative strain impaired in mangotoxin production also showed a reduction in virulence as measured by necrotic symptoms on tomato leaflets. Mangotoxin production and virulence were restored fully in the NRPS mutant by complementation with plasmid pCG2-6, which contains an 11,103-bp chromosomal region cloned from the wildtype strain P. syringae pv. syringae UMAF0158 that includes the putative NPRS gene (mgoA). The results demonstrate that mgoA has a role in the virulence of P. syringae pv. syringae. The involvement of an NRPS in the production of an antimetabolite toxin from P. syringae inhibiting ornithine acetyltransferase activity is proposed. Arrebola E, Cazorla FM, Romero D, Pérez-García A, de Vicente A. A nonribosomal peptide synthetase gene (mgoA) of Pseudomonas syringae pv. syringae is involved in mangotoxin biosynthesis and is required for full virulence. Mol Plant Microbe Interact. 2007. 20(5):500-9. https://hdl.handle.net/10630/30984 10.1094/MPMI-20-5-0500. Fitotoxinas Mangos - Enfermedades y plagas Antimetabolitos Bacterias fitopatógenas A Nonribosomal Peptide Synthetase Gene (mgoA) of Pseudomonas syringae pv. syringae Is Involved in Mangotoxin Biosynthesis and Is Required for Full Virulence.