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oai:riuma.uma.es:10630/311342026-02-03T11:26:55Zcom_10630_2254col_10630_37953

Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA. Carrión Bravo, Víctor José Van der Voort, Menno Arrebola-Díez, Eva María Gutiérrez Barranquero, José Antonio De-Vicente-Moreno, Antonio Raaijmakers, Jos M. Cazorla-López, Francisco Manuel Fitotoxinas Antimetabolitos Bacterias fitopatógenas Operones Background: The antimetabolite mangotoxin is a key factor in virulence of Pseudomonas syringae pv. syringae strains which cause apical necrosis of mango trees. Previous studies showed that mangotoxin biosynthesis is governed by the mbo operon. Random mutagenesis led to the identification of two other gene clusters that affect mangotoxin biosynthesis. These are the gacS/gacA genes and mgo operon which harbors the four genes mgoBCAD. Results: The current study shows that disruption of the nonribosomal peptide synthetase (NRPS) gene mgoA resulted in loss of mangotoxin production and reduced virulence on tomato leaves. Transcriptional analyses by qPCR and promoter reporter fusions revealed that mbo expression is regulated by both gacS/gacA and mgo genes. Also, expression of the mgo operon was shown to be regulated by gacS/gacA. Heterologous expression under the native promoter of the mbo operon resulted in mangotoxin production in non-producing P. syringae strains, but not in other Pseudomonas species. Also introduction of the mbo and mgo operons in nonproducing P. protegens Pf-5 did not confer mangotoxin production but did enhance transcription of the mbo promoter. Conclusions: From the data obtained in this study, we conclude that both mbo and mgo operons are under the control of the gacS/gacA two-component system and that the MgoA product acts as a positive regulator of mangotoxin biosynthesis. 2024-04-23T10:09:43Z 2024-04-23T10:09:43Z 2024-04-23T10:09:43Z 2014-02-21 journal article Carrión VJ, van der Voort M, Arrebola E, Gutiérrez-Barranquero JA, de Vicente A, Raaijmakers JM, Cazorla FM. Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA. BMC Microbiol. 2014 Feb 21;14:46. https://hdl.handle.net/10630/31134 10.1186/1471-2180-14-46 eng http://creativecommons.org/licenses/by/4.0/ open access Attribution 4.0 Internacional BMC